Mapping of 53 Loci in American Mink (Mustela vison)
Fifty-three genes were mapped in the American mink genome using polymerase chain reaction (PCR)-based analysis of a Chinese hamster–American mink somatic cell hybrid panel. Heterologous primers designed for cat gene mapping were used in this study. Forty-nine of these loci were localized into expect...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Text |
| Language: | English |
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| Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.602.7872 http://jhered.oxfordjournals.org/content/94/5/386.full.pdf |
| Summary: | Fifty-three genes were mapped in the American mink genome using polymerase chain reaction (PCR)-based analysis of a Chinese hamster–American mink somatic cell hybrid panel. Heterologous primers designed for cat gene mapping were used in this study. Forty-nine of these loci were localized into expected chromosome regions according to Zoo-FISH data, whereas four loci—ALPL, CDC20, ERF-2, and Fc(Mv)23617—were mapped out of expected conserved regions. PCR products amplified with primers corresponding to these four markers were partly sequenced and verified using BLAST. The results showed the homology to be more than 90 % between mink and human or cat counterparts. At present, the gene map of American mink has expanded to 127 loci. The American mink (Mustela vison) is a representative of the large family Mustelidae belonging to the suborder Caniformia in the order Carnivora. Carnivores include hundreds of species living in a broad range of geographic zones from the Arctic to Antarctica, and they are of great interest in comparative gene mapping because they have complex evolution and phylogeny. Moreover, gene mapping in carnivore species |
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