Summary
We have measured the metabolic activity in the vascularly isolated, saline-perfused swimbladder of the eel (Anguilla anguilla) in order to investigate the pathways for CO2 formation in the gas gland tissue. Concentrations of O2, CO2, glucose and lactate were measured in the arterial inflow and venou...
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1989
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.600.3647 2023-05-15T13:27:45+02:00 Summary B. Pelster H. Kobayashi P. Scheid The Pennsylvania State University CiteSeerX Archives 1989 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.600.3647 http://jeb.biologists.org/content/144/1/495.full.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.600.3647 http://jeb.biologists.org/content/144/1/495.full.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://jeb.biologists.org/content/144/1/495.full.pdf text 1989 ftciteseerx 2016-01-08T14:00:42Z We have measured the metabolic activity in the vascularly isolated, saline-perfused swimbladder of the eel (Anguilla anguilla) in order to investigate the pathways for CO2 formation in the gas gland tissue. Concentrations of O2, CO2, glucose and lactate were measured in the arterial inflow and venous outflow of the swimbladder, and metabolic rates were calculated by the direct Fick principle. 1. Total CO2 production, averaging 55-Snmolmin"1, was about 4-6 times the O2 consumption (mean 12-0nmolmin~1). This suggests that only about 22 % of the CO2 is formed by aerobic glucose metabolism. 2. CO2 formation from HCO3 ~ or CO2 washout does not appear to be significant in our experiments with steady perfusion of a saline containing a low level of HCO3-. 3. The ratio of lactate production to glucose uptake averaged 1-2, indicating that only 60 % of the glucose is converted to lactate. Since only 1-2 % of the glucose was found to be oxidized (2nmolmin~1), the extra glucose appears to be anoxidatively metabolized to CO2. 4. The anoxidative CO2 formation appears to be of functional importance for producing the high gas partial pressures of both CO2 and O2 which are required for secretion of these gases into the swimbladder. Text Anguilla anguilla Unknown |
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English |
description |
We have measured the metabolic activity in the vascularly isolated, saline-perfused swimbladder of the eel (Anguilla anguilla) in order to investigate the pathways for CO2 formation in the gas gland tissue. Concentrations of O2, CO2, glucose and lactate were measured in the arterial inflow and venous outflow of the swimbladder, and metabolic rates were calculated by the direct Fick principle. 1. Total CO2 production, averaging 55-Snmolmin"1, was about 4-6 times the O2 consumption (mean 12-0nmolmin~1). This suggests that only about 22 % of the CO2 is formed by aerobic glucose metabolism. 2. CO2 formation from HCO3 ~ or CO2 washout does not appear to be significant in our experiments with steady perfusion of a saline containing a low level of HCO3-. 3. The ratio of lactate production to glucose uptake averaged 1-2, indicating that only 60 % of the glucose is converted to lactate. Since only 1-2 % of the glucose was found to be oxidized (2nmolmin~1), the extra glucose appears to be anoxidatively metabolized to CO2. 4. The anoxidative CO2 formation appears to be of functional importance for producing the high gas partial pressures of both CO2 and O2 which are required for secretion of these gases into the swimbladder. |
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The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
B. Pelster H. Kobayashi P. Scheid |
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B. Pelster H. Kobayashi P. Scheid Summary |
author_facet |
B. Pelster H. Kobayashi P. Scheid |
author_sort |
B. Pelster |
title |
Summary |
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title_full |
Summary |
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publishDate |
1989 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.600.3647 http://jeb.biologists.org/content/144/1/495.full.pdf |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
http://jeb.biologists.org/content/144/1/495.full.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.600.3647 http://jeb.biologists.org/content/144/1/495.full.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766400219550842880 |