Article Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate
Abstract: The pacific oyster Crassostrea gigas was experimentally exposed to the neurotoxic Alexandrium catenella and a non-producer of PSTs, Alexandrium tamarense (control algae), at concentrations corresponding to those observed during the blooming period. At fixed time intervals, from 0 to 48 h,...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.591.8874 2023-05-15T15:57:53+02:00 Article Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium Catenella Jean-luc Rolland Kevin Pelletier Veronique Savar Adrien Santini Zouher Amzil Mohamed Laabir The Pennsylvania State University CiteSeerX Archives 2012 http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.591.8874 http://www.mdpi.com/1660-3397/10/11/2519/pdf/ en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.591.8874 http://www.mdpi.com/1660-3397/10/11/2519/pdf/ Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.mdpi.com/1660-3397/10/11/2519/pdf/ text 2012 ftciteseerx 2016-01-08T13:33:53Z Abstract: The pacific oyster Crassostrea gigas was experimentally exposed to the neurotoxic Alexandrium catenella and a non-producer of PSTs, Alexandrium tamarense (control algae), at concentrations corresponding to those observed during the blooming period. At fixed time intervals, from 0 to 48 h, we determined the clearance rate, the total filtered cells, the composition of the fecal ribbons, the profile of the PSP toxins and the variation of the expression of two α-amylase and triacylglecerol lipase precursor (TLP) genes through semi-quantitative RT-PCR. The results showed a significant decrease of the clearance rate of C. gigas fed with both Alexandrium species. However, from 29 to 48 h, the clearance rate and cell filtration activity increased only in oysters fed with A. tamarense. The toxin concentrations in the digestive gland rose above the sanitary threshold in less than 48 h of exposure and GTX6, a compound absent in A. catenella cells, accumulated. The α-amylase B gene expression level increased significantly in the time Text Crassostrea gigas Pacific oyster Unknown Pacific |
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Open Polar |
collection |
Unknown |
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ftciteseerx |
language |
English |
description |
Abstract: The pacific oyster Crassostrea gigas was experimentally exposed to the neurotoxic Alexandrium catenella and a non-producer of PSTs, Alexandrium tamarense (control algae), at concentrations corresponding to those observed during the blooming period. At fixed time intervals, from 0 to 48 h, we determined the clearance rate, the total filtered cells, the composition of the fecal ribbons, the profile of the PSP toxins and the variation of the expression of two α-amylase and triacylglecerol lipase precursor (TLP) genes through semi-quantitative RT-PCR. The results showed a significant decrease of the clearance rate of C. gigas fed with both Alexandrium species. However, from 29 to 48 h, the clearance rate and cell filtration activity increased only in oysters fed with A. tamarense. The toxin concentrations in the digestive gland rose above the sanitary threshold in less than 48 h of exposure and GTX6, a compound absent in A. catenella cells, accumulated. The α-amylase B gene expression level increased significantly in the time |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Alexandrium Catenella Jean-luc Rolland Kevin Pelletier Veronique Savar Adrien Santini Zouher Amzil Mohamed Laabir |
spellingShingle |
Alexandrium Catenella Jean-luc Rolland Kevin Pelletier Veronique Savar Adrien Santini Zouher Amzil Mohamed Laabir Article Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate |
author_facet |
Alexandrium Catenella Jean-luc Rolland Kevin Pelletier Veronique Savar Adrien Santini Zouher Amzil Mohamed Laabir |
author_sort |
Alexandrium Catenella |
title |
Article Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate |
title_short |
Article Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate |
title_full |
Article Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate |
title_fullStr |
Article Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate |
title_full_unstemmed |
Article Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate |
title_sort |
article paralytic toxins accumulation and tissue expression of α-amylase and lipase genes in the pacific oyster crassostrea gigas fed with the neurotoxic dinoflagellate |
publishDate |
2012 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.591.8874 http://www.mdpi.com/1660-3397/10/11/2519/pdf/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
http://www.mdpi.com/1660-3397/10/11/2519/pdf/ |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.591.8874 http://www.mdpi.com/1660-3397/10/11/2519/pdf/ |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766393588652965888 |