Purification and partial genome characterization of a herpes- like virus infecting the Japanese oyster, Crassostrea gigas
First observed in 1972 in Crassostrea virginica, herpes-like viruses of bivalves were more recently found to be associated with high mortality rates in other cultured oyster species, such as Crassostrea gigas and Ostrea edulis. The diagnosis of herpes-like virus infections is performed currently by...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.588.9034 2023-05-15T15:57:53+02:00 Purification and partial genome characterization of a herpes- like virus infecting the Japanese oyster, Crassostrea gigas Rose-marie Le Deuff Tristan Renault The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.588.9034 http://vir.sgmjournals.org/content/80/5/1317.full.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.588.9034 http://vir.sgmjournals.org/content/80/5/1317.full.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://vir.sgmjournals.org/content/80/5/1317.full.pdf text ftciteseerx 2016-01-08T13:23:22Z First observed in 1972 in Crassostrea virginica, herpes-like viruses of bivalves were more recently found to be associated with high mortality rates in other cultured oyster species, such as Crassostrea gigas and Ostrea edulis. The diagnosis of herpes-like virus infections is performed currently by laborious histological and transmission electron microscope examinations. Prep-aration of specific reagents for use in more amenable diagnostic techniques prompted purification of virus particles and investigation of the viral genome. This paper is the first description of the purification of a virus pathogen from a bivalve mollusc. A procedure was developed which facilitated purification of large amounts of virus particles on the 40–50 % interface of sucrose gradients. Transmission electron microscopy showed that a purified virus suspension contained capsids and enveloped virus particles. High molecular mass viral DNA was extracted, and the genome size was estimated by the summation of the sizes of restriction endonuclease fragments to be approximately 180 kbp. Partial cloning of the virus genome was achieved and the specificity of certain cloned fragments was established by dot blot hybridization. Text Crassostrea gigas Unknown |
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ftciteseerx |
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English |
description |
First observed in 1972 in Crassostrea virginica, herpes-like viruses of bivalves were more recently found to be associated with high mortality rates in other cultured oyster species, such as Crassostrea gigas and Ostrea edulis. The diagnosis of herpes-like virus infections is performed currently by laborious histological and transmission electron microscope examinations. Prep-aration of specific reagents for use in more amenable diagnostic techniques prompted purification of virus particles and investigation of the viral genome. This paper is the first description of the purification of a virus pathogen from a bivalve mollusc. A procedure was developed which facilitated purification of large amounts of virus particles on the 40–50 % interface of sucrose gradients. Transmission electron microscopy showed that a purified virus suspension contained capsids and enveloped virus particles. High molecular mass viral DNA was extracted, and the genome size was estimated by the summation of the sizes of restriction endonuclease fragments to be approximately 180 kbp. Partial cloning of the virus genome was achieved and the specificity of certain cloned fragments was established by dot blot hybridization. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Rose-marie Le Deuff Tristan Renault |
spellingShingle |
Rose-marie Le Deuff Tristan Renault Purification and partial genome characterization of a herpes- like virus infecting the Japanese oyster, Crassostrea gigas |
author_facet |
Rose-marie Le Deuff Tristan Renault |
author_sort |
Rose-marie Le Deuff |
title |
Purification and partial genome characterization of a herpes- like virus infecting the Japanese oyster, Crassostrea gigas |
title_short |
Purification and partial genome characterization of a herpes- like virus infecting the Japanese oyster, Crassostrea gigas |
title_full |
Purification and partial genome characterization of a herpes- like virus infecting the Japanese oyster, Crassostrea gigas |
title_fullStr |
Purification and partial genome characterization of a herpes- like virus infecting the Japanese oyster, Crassostrea gigas |
title_full_unstemmed |
Purification and partial genome characterization of a herpes- like virus infecting the Japanese oyster, Crassostrea gigas |
title_sort |
purification and partial genome characterization of a herpes- like virus infecting the japanese oyster, crassostrea gigas |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.588.9034 http://vir.sgmjournals.org/content/80/5/1317.full.pdf |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
http://vir.sgmjournals.org/content/80/5/1317.full.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.588.9034 http://vir.sgmjournals.org/content/80/5/1317.full.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766393587425083392 |