Effect of nitrogen enrichment under different concentrations of silicate on the relative growth of ice algae in resolute passage, Canadian arctic (extended abstract

• Main Authors: Satoru Taguchi, Ralph E. H. Smith
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Published: 1995
• Subjects: Antarctic; Arctic; Resolute Passage; The Antarctic; Antarc*; ice algae; Subarctic
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.578.4839; http://polaris.nipr.ac.jp/~penguin/polarbiosci/issues/pdf/1995-Taguchi2.pdf

Nitrogen nutrition of ice algae is still controversial although studies have been conducted in both the Antarctic (COTA et a/., 1990; PRISCU et al., 1989, 199 I) , and the Arctic (HARRISON et al., 1990) and subarctic (DEMERS et al., 1989). In the present study photosynthate allocation was determined to study nitrogen nutrition of ice algae. Enrichment experiments with ammonium, nitrate, and silicate were conducted to determine their effect on the end products of photosynthesis with ice alga collected from Resolute Passage, Canadian Arctic during the summer of 1992. End products of photosynthesis were determined by the method described by SMITH et al. (1987). It is conventional to refer to the four resulting fractions as follows: methanol-water = low molecular weight compounds (LMWC), chloroform-soluble = lipid, TCA- soluble = polysaccharide with nucleic acids, and TCA-insoluble=protein. The samples of lipid fraction were further analyzed for four lipid classes including triacyglycerols (TG), free fatty acids (FFA), acetone-mobile polar lipids (AMPL), and phospholipids (PL) by the method described by SMITH