Environmental Microbiology (2001) 3(9), 570–577 Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice
Lake Vostok, the largest subglacial lake in Antarctica, is separated from the surface by < 4 km of glacial ice. It has been isolated from direct surface input for at least 420 000 years, and the possibility of a novel environment and ecosystem therefore exists. Lake Vostok water has not been samp...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.566.3712 2023-05-15T13:38:55+02:00 Environmental Microbiology (2001) 3(9), 570–577 Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice Brent C. Christner Ellen Mosley-thompson Lonnie G. Thompson John N. Reeve The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.566.3712 http://salegos-scar.montana.edu/docs/Literaturedocs/Bacteriaisolates/Bacteriaisolates.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.566.3712 http://salegos-scar.montana.edu/docs/Literaturedocs/Bacteriaisolates/Bacteriaisolates.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://salegos-scar.montana.edu/docs/Literaturedocs/Bacteriaisolates/Bacteriaisolates.pdf text ftciteseerx 2016-01-08T12:16:49Z Lake Vostok, the largest subglacial lake in Antarctica, is separated from the surface by < 4 km of glacial ice. It has been isolated from direct surface input for at least 420 000 years, and the possibility of a novel environment and ecosystem therefore exists. Lake Vostok water has not been sampled, but an ice core has been recovered that extends into the ice accreted below glacial ice by freezing of Lake Vostok water. Here, we report the recovery of bacterial isolates belonging to the Brachybacteria, Methylobacterium, Paenibacillus and Sphingomonas lineages from a sample of melt water from this accretion ice that originated 3593 m below the surface. We have also amplified small-subunit ribosomal RNA-encoding DNA molecules (16S rDNAs) directly from this melt water that originated from a- and b-proteobacteria, low- and high-G1C Gram-positive bacteria and a member of the Cytophaga/Flavobacterium/Bacteroides lineage. Text Antarc* Antarctica ice core Unknown Lake Vostok ENVELOPE(106.000,106.000,-77.500,-77.500) |
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ftciteseerx |
language |
English |
description |
Lake Vostok, the largest subglacial lake in Antarctica, is separated from the surface by < 4 km of glacial ice. It has been isolated from direct surface input for at least 420 000 years, and the possibility of a novel environment and ecosystem therefore exists. Lake Vostok water has not been sampled, but an ice core has been recovered that extends into the ice accreted below glacial ice by freezing of Lake Vostok water. Here, we report the recovery of bacterial isolates belonging to the Brachybacteria, Methylobacterium, Paenibacillus and Sphingomonas lineages from a sample of melt water from this accretion ice that originated 3593 m below the surface. We have also amplified small-subunit ribosomal RNA-encoding DNA molecules (16S rDNAs) directly from this melt water that originated from a- and b-proteobacteria, low- and high-G1C Gram-positive bacteria and a member of the Cytophaga/Flavobacterium/Bacteroides lineage. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Brent C. Christner Ellen Mosley-thompson Lonnie G. Thompson John N. Reeve |
spellingShingle |
Brent C. Christner Ellen Mosley-thompson Lonnie G. Thompson John N. Reeve Environmental Microbiology (2001) 3(9), 570–577 Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice |
author_facet |
Brent C. Christner Ellen Mosley-thompson Lonnie G. Thompson John N. Reeve |
author_sort |
Brent C. Christner |
title |
Environmental Microbiology (2001) 3(9), 570–577 Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice |
title_short |
Environmental Microbiology (2001) 3(9), 570–577 Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice |
title_full |
Environmental Microbiology (2001) 3(9), 570–577 Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice |
title_fullStr |
Environmental Microbiology (2001) 3(9), 570–577 Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice |
title_full_unstemmed |
Environmental Microbiology (2001) 3(9), 570–577 Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice |
title_sort |
environmental microbiology (2001) 3(9), 570–577 isolation of bacteria and 16s rdnas from lake vostok accretion ice |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.566.3712 http://salegos-scar.montana.edu/docs/Literaturedocs/Bacteriaisolates/Bacteriaisolates.pdf |
long_lat |
ENVELOPE(106.000,106.000,-77.500,-77.500) |
geographic |
Lake Vostok |
geographic_facet |
Lake Vostok |
genre |
Antarc* Antarctica ice core |
genre_facet |
Antarc* Antarctica ice core |
op_source |
http://salegos-scar.montana.edu/docs/Literaturedocs/Bacteriaisolates/Bacteriaisolates.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.566.3712 http://salegos-scar.montana.edu/docs/Literaturedocs/Bacteriaisolates/Bacteriaisolates.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766112497918541824 |