Research Article Laser-Induced Fluorescence Emission (L.I.F.E.): In Situ Nondestructive Detection of Microbial Life in the Ice Covers of Antarctic Lakes
Laser-induced fluorescence emission (L.I.F.E.) images were obtained in situ following 532 nm excitation of cryoconite assemblages in the ice covers of annual and perennially frozen Antarctic lakes during the 2008 Tawani International Expedition to Schirmacher Oasis and Lake Untersee in Dronning Maud...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.556.6401 2023-05-15T13:59:20+02:00 Research Article Laser-Induced Fluorescence Emission (L.I.F.E.): In Situ Nondestructive Detection of Microbial Life in the Ice Covers of Antarctic Lakes Michael C. Storrie-lombardi Birgit Sattler The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.556.6401 http://expedition.tawanifoundation.org/wp-content/themes/brightness theme/files/untersee_life.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.556.6401 http://expedition.tawanifoundation.org/wp-content/themes/brightness theme/files/untersee_life.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://expedition.tawanifoundation.org/wp-content/themes/brightness theme/files/untersee_life.pdf text ftciteseerx 2016-01-08T11:48:27Z Laser-induced fluorescence emission (L.I.F.E.) images were obtained in situ following 532 nm excitation of cryoconite assemblages in the ice covers of annual and perennially frozen Antarctic lakes during the 2008 Tawani International Expedition to Schirmacher Oasis and Lake Untersee in Dronning Maud Land, Antarctica. Laser targeting of a single millimeter-scale cryoconite results in multiple neighboring excitation events secondary to ice=air interface reflection and refraction in the bubbles surrounding the primary target. Laser excitation at 532 nm of cyanobacteria-dominated assemblages produced red and infrared autofluorescence activity attributed to the presence of phycoerythrin photosynthetic pigments. The method avoids destruction of individual target organisms and does not require the disruption of either the structure of the microbial community or the surrounding ice matrix. L.I.F.E. survey strategies described may be of interest for orbital monitoring of photo-synthetic primary productivity in polar and alpine glaciers, ice sheets, snow, and lake ice of Earth’s cryosphere. The findings open up the possibility of searching from either a rover or from orbit for signs of life in the polar regions of Mars and the frozen regions of exoplanets in neighboring star systems. Key Words: Cryosphere— Ice—Mars—Photosynthesis—Psychrophiles. Astrobiology 9, 659–672. Text Antarc* Antarctic Antarctica Dronning Maud Land Unknown Antarctic Dronning Maud Land Untersee ENVELOPE(13.467,13.467,-71.350,-71.350) |
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English |
description |
Laser-induced fluorescence emission (L.I.F.E.) images were obtained in situ following 532 nm excitation of cryoconite assemblages in the ice covers of annual and perennially frozen Antarctic lakes during the 2008 Tawani International Expedition to Schirmacher Oasis and Lake Untersee in Dronning Maud Land, Antarctica. Laser targeting of a single millimeter-scale cryoconite results in multiple neighboring excitation events secondary to ice=air interface reflection and refraction in the bubbles surrounding the primary target. Laser excitation at 532 nm of cyanobacteria-dominated assemblages produced red and infrared autofluorescence activity attributed to the presence of phycoerythrin photosynthetic pigments. The method avoids destruction of individual target organisms and does not require the disruption of either the structure of the microbial community or the surrounding ice matrix. L.I.F.E. survey strategies described may be of interest for orbital monitoring of photo-synthetic primary productivity in polar and alpine glaciers, ice sheets, snow, and lake ice of Earth’s cryosphere. The findings open up the possibility of searching from either a rover or from orbit for signs of life in the polar regions of Mars and the frozen regions of exoplanets in neighboring star systems. Key Words: Cryosphere— Ice—Mars—Photosynthesis—Psychrophiles. Astrobiology 9, 659–672. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Michael C. Storrie-lombardi Birgit Sattler |
spellingShingle |
Michael C. Storrie-lombardi Birgit Sattler Research Article Laser-Induced Fluorescence Emission (L.I.F.E.): In Situ Nondestructive Detection of Microbial Life in the Ice Covers of Antarctic Lakes |
author_facet |
Michael C. Storrie-lombardi Birgit Sattler |
author_sort |
Michael C. Storrie-lombardi |
title |
Research Article Laser-Induced Fluorescence Emission (L.I.F.E.): In Situ Nondestructive Detection of Microbial Life in the Ice Covers of Antarctic Lakes |
title_short |
Research Article Laser-Induced Fluorescence Emission (L.I.F.E.): In Situ Nondestructive Detection of Microbial Life in the Ice Covers of Antarctic Lakes |
title_full |
Research Article Laser-Induced Fluorescence Emission (L.I.F.E.): In Situ Nondestructive Detection of Microbial Life in the Ice Covers of Antarctic Lakes |
title_fullStr |
Research Article Laser-Induced Fluorescence Emission (L.I.F.E.): In Situ Nondestructive Detection of Microbial Life in the Ice Covers of Antarctic Lakes |
title_full_unstemmed |
Research Article Laser-Induced Fluorescence Emission (L.I.F.E.): In Situ Nondestructive Detection of Microbial Life in the Ice Covers of Antarctic Lakes |
title_sort |
research article laser-induced fluorescence emission (l.i.f.e.): in situ nondestructive detection of microbial life in the ice covers of antarctic lakes |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.556.6401 http://expedition.tawanifoundation.org/wp-content/themes/brightness theme/files/untersee_life.pdf |
long_lat |
ENVELOPE(13.467,13.467,-71.350,-71.350) |
geographic |
Antarctic Dronning Maud Land Untersee |
geographic_facet |
Antarctic Dronning Maud Land Untersee |
genre |
Antarc* Antarctic Antarctica Dronning Maud Land |
genre_facet |
Antarc* Antarctic Antarctica Dronning Maud Land |
op_source |
http://expedition.tawanifoundation.org/wp-content/themes/brightness theme/files/untersee_life.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.556.6401 http://expedition.tawanifoundation.org/wp-content/themes/brightness theme/files/untersee_life.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766267871909904384 |