The Use of Semipermeable Membrane Devices (SPMDs) to Concentrate Inducers of Fish Hepatic Mixed Function Oxygenase (MFO)
Semipermeable membrane devices (SPMDs) are sampling and concentrating devices comprised of a thin polyethylene membiane containing a small quantity of triolein. They have previously been used to sample air, water and sediments and have concentrated fish tainting compounds from pulp mill effluents. T...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.550.4103 2023-05-15T15:26:04+02:00 The Use of Semipermeable Membrane Devices (SPMDs) to Concentrate Inducers of Fish Hepatic Mixed Function Oxygenase (MFO) Joanne L. Parrott Donald E. Tillitt The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.550.4103 http://www.ecrc.cr.usgs.gov/pubs/center/pdfDocs/90906.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.550.4103 http://www.ecrc.cr.usgs.gov/pubs/center/pdfDocs/90906.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.ecrc.cr.usgs.gov/pubs/center/pdfDocs/90906.pdf text ftciteseerx 2016-01-08T11:31:46Z Semipermeable membrane devices (SPMDs) are sampling and concentrating devices comprised of a thin polyethylene membiane containing a small quantity of triolein. They have previously been used to sample air, water and sediments and have concentrated fish tainting compounds from pulp mill effluents. The ability to induce mixed function oxygenases (MFOs) is a property of a variety of organic effluents, but the compound(s) responsible for induction have not been identified. We wanted to see if SPMDs would accumulate the MFO-inducing chemical(s) from pulp mill effluents and oil refinery effluents. Dialysates of effluent-exposed SPMDs induced ethoxyresorufin-0-deethylase (EROD) activity in a fish (Poeciliopsis lucida) hepatoma cell line, PLHC-1. In pulp mill effluents and oil sands mining and refining wastewaters, potencies varied greatly, from a few to thousands of pg TCDD-EQ/g SPMD. Low levels of inducers were seen in four pulp mills on the Athabasca R., and higher levels at one New Brunswick bleached sulphite and two Ontario bleached kraft pulp mills. The highest levels of MFO inducers were in SPMDs deployed for 14 days in wastewater from an oil sands upgrading facility, as well as SPMDs deployed at two sites on Athabasca River tributaries in the oil sands area. This suggests that natural erosion and weathering, as well as industrial processing of the Text Athabasca River Unknown Athabasca River |
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description |
Semipermeable membrane devices (SPMDs) are sampling and concentrating devices comprised of a thin polyethylene membiane containing a small quantity of triolein. They have previously been used to sample air, water and sediments and have concentrated fish tainting compounds from pulp mill effluents. The ability to induce mixed function oxygenases (MFOs) is a property of a variety of organic effluents, but the compound(s) responsible for induction have not been identified. We wanted to see if SPMDs would accumulate the MFO-inducing chemical(s) from pulp mill effluents and oil refinery effluents. Dialysates of effluent-exposed SPMDs induced ethoxyresorufin-0-deethylase (EROD) activity in a fish (Poeciliopsis lucida) hepatoma cell line, PLHC-1. In pulp mill effluents and oil sands mining and refining wastewaters, potencies varied greatly, from a few to thousands of pg TCDD-EQ/g SPMD. Low levels of inducers were seen in four pulp mills on the Athabasca R., and higher levels at one New Brunswick bleached sulphite and two Ontario bleached kraft pulp mills. The highest levels of MFO inducers were in SPMDs deployed for 14 days in wastewater from an oil sands upgrading facility, as well as SPMDs deployed at two sites on Athabasca River tributaries in the oil sands area. This suggests that natural erosion and weathering, as well as industrial processing of the |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Joanne L. Parrott Donald E. Tillitt |
spellingShingle |
Joanne L. Parrott Donald E. Tillitt The Use of Semipermeable Membrane Devices (SPMDs) to Concentrate Inducers of Fish Hepatic Mixed Function Oxygenase (MFO) |
author_facet |
Joanne L. Parrott Donald E. Tillitt |
author_sort |
Joanne L. Parrott |
title |
The Use of Semipermeable Membrane Devices (SPMDs) to Concentrate Inducers of Fish Hepatic Mixed Function Oxygenase (MFO) |
title_short |
The Use of Semipermeable Membrane Devices (SPMDs) to Concentrate Inducers of Fish Hepatic Mixed Function Oxygenase (MFO) |
title_full |
The Use of Semipermeable Membrane Devices (SPMDs) to Concentrate Inducers of Fish Hepatic Mixed Function Oxygenase (MFO) |
title_fullStr |
The Use of Semipermeable Membrane Devices (SPMDs) to Concentrate Inducers of Fish Hepatic Mixed Function Oxygenase (MFO) |
title_full_unstemmed |
The Use of Semipermeable Membrane Devices (SPMDs) to Concentrate Inducers of Fish Hepatic Mixed Function Oxygenase (MFO) |
title_sort |
use of semipermeable membrane devices (spmds) to concentrate inducers of fish hepatic mixed function oxygenase (mfo) |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.550.4103 http://www.ecrc.cr.usgs.gov/pubs/center/pdfDocs/90906.pdf |
geographic |
Athabasca River |
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Athabasca River |
genre |
Athabasca River |
genre_facet |
Athabasca River |
op_source |
http://www.ecrc.cr.usgs.gov/pubs/center/pdfDocs/90906.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.550.4103 http://www.ecrc.cr.usgs.gov/pubs/center/pdfDocs/90906.pdf |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766356636127985664 |