KINETIC PROPERTIES OF PYRUVATE KINASE PURIFIED FROM THE FLESH MUSCLE OF THE ANTARCTIC KRILL EUPHA USIA SUPERBA DANA*
Abstract: In previous experiments, BACILA, ROSA, and RODRIGUES showed that enolase, partially purified from the flesh muscle of the krill Euphausia superba DANA, has not been inhibited by F in concentrations as high as 2 mM, a property which is paralleled by the enolase from the penguins liver. This...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.525.9077 2023-05-15T13:37:27+02:00 KINETIC PROPERTIES OF PYRUVATE KINASE PURIFIED FROM THE FLESH MUSCLE OF THE ANTARCTIC KRILL EUPHA USIA SUPERBA DANA* Edson Rodrigues Metry Bacila The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.525.9077 http://polaris.nipr.ac.jp/~penguin/polarbiosci/issues/pdf/1994-Rosa.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.525.9077 http://polaris.nipr.ac.jp/~penguin/polarbiosci/issues/pdf/1994-Rosa.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://polaris.nipr.ac.jp/~penguin/polarbiosci/issues/pdf/1994-Rosa.pdf text ftciteseerx 2016-01-08T10:21:26Z Abstract: In previous experiments, BACILA, ROSA, and RODRIGUES showed that enolase, partially purified from the flesh muscle of the krill Euphausia superba DANA, has not been inhibited by F in concentrations as high as 2 mM, a property which is paralleled by the enolase from the penguins liver. This finding led to the study of the structure and the kinetic properties of other enzymes from the carbohydrate metabolism of the krill's flesh muscle. In the present communication some data on the kinetic properties of the pyruvate kinase (PK) purified from the krill's flesh muscle are shown. L-Phenylalanine inhibits this preparation of PK both in the presence and in the absence of 1 mM L-alanine. However, the inhibition of PK by Mg-ATP was almost completely reversed in the presence of 1 mM Fru-P2, but not in the presence of 1 mM L-alanine. The Km values for PK have been established to be 0.03 pM PEP and 0.038 pM PEP respectively in the presence and in the absence of Fru-Pz. This flesh muscle krill's preparation of PK shows clear sigmoidal kinetics. However, in the presence of Fru-P2, PK shows a Michaelian kinetics. The effect of ADP has also been established and found to have a Km value of 0.15 pM ADP. Cations inhibit the krill's pyruvate kinase in the following order: M n 2> C a 2> Ni2+> Co2+ while Li2+ did not show any inhibitory effect. 1. Text Antarc* Antarctic Antarctic Krill Euphausia superba Unknown Antarctic The Antarctic |
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Unknown |
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ftciteseerx |
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English |
description |
Abstract: In previous experiments, BACILA, ROSA, and RODRIGUES showed that enolase, partially purified from the flesh muscle of the krill Euphausia superba DANA, has not been inhibited by F in concentrations as high as 2 mM, a property which is paralleled by the enolase from the penguins liver. This finding led to the study of the structure and the kinetic properties of other enzymes from the carbohydrate metabolism of the krill's flesh muscle. In the present communication some data on the kinetic properties of the pyruvate kinase (PK) purified from the krill's flesh muscle are shown. L-Phenylalanine inhibits this preparation of PK both in the presence and in the absence of 1 mM L-alanine. However, the inhibition of PK by Mg-ATP was almost completely reversed in the presence of 1 mM Fru-P2, but not in the presence of 1 mM L-alanine. The Km values for PK have been established to be 0.03 pM PEP and 0.038 pM PEP respectively in the presence and in the absence of Fru-Pz. This flesh muscle krill's preparation of PK shows clear sigmoidal kinetics. However, in the presence of Fru-P2, PK shows a Michaelian kinetics. The effect of ADP has also been established and found to have a Km value of 0.15 pM ADP. Cations inhibit the krill's pyruvate kinase in the following order: M n 2> C a 2> Ni2+> Co2+ while Li2+ did not show any inhibitory effect. 1. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Edson Rodrigues Metry Bacila |
spellingShingle |
Edson Rodrigues Metry Bacila KINETIC PROPERTIES OF PYRUVATE KINASE PURIFIED FROM THE FLESH MUSCLE OF THE ANTARCTIC KRILL EUPHA USIA SUPERBA DANA* |
author_facet |
Edson Rodrigues Metry Bacila |
author_sort |
Edson Rodrigues |
title |
KINETIC PROPERTIES OF PYRUVATE KINASE PURIFIED FROM THE FLESH MUSCLE OF THE ANTARCTIC KRILL EUPHA USIA SUPERBA DANA* |
title_short |
KINETIC PROPERTIES OF PYRUVATE KINASE PURIFIED FROM THE FLESH MUSCLE OF THE ANTARCTIC KRILL EUPHA USIA SUPERBA DANA* |
title_full |
KINETIC PROPERTIES OF PYRUVATE KINASE PURIFIED FROM THE FLESH MUSCLE OF THE ANTARCTIC KRILL EUPHA USIA SUPERBA DANA* |
title_fullStr |
KINETIC PROPERTIES OF PYRUVATE KINASE PURIFIED FROM THE FLESH MUSCLE OF THE ANTARCTIC KRILL EUPHA USIA SUPERBA DANA* |
title_full_unstemmed |
KINETIC PROPERTIES OF PYRUVATE KINASE PURIFIED FROM THE FLESH MUSCLE OF THE ANTARCTIC KRILL EUPHA USIA SUPERBA DANA* |
title_sort |
kinetic properties of pyruvate kinase purified from the flesh muscle of the antarctic krill eupha usia superba dana* |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.525.9077 http://polaris.nipr.ac.jp/~penguin/polarbiosci/issues/pdf/1994-Rosa.pdf |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba |
op_source |
http://polaris.nipr.ac.jp/~penguin/polarbiosci/issues/pdf/1994-Rosa.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.525.9077 http://polaris.nipr.ac.jp/~penguin/polarbiosci/issues/pdf/1994-Rosa.pdf |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766091950591574016 |