Contributed papers Time should be considered in developmental ecotoxicity test
Developmental toxicity tests are often used for the hazard assessment of chemicals and environmental media. One of the most widely used is the oyster embryo larval test (OEL), in which the development of oyster larvae is arrested at a single fixed time (e.g. 24 or 48 h) of toxic exposure, and the pr...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.523.795 2023-05-15T15:58:48+02:00 Contributed papers Time should be considered in developmental ecotoxicity test Mathew A. Worboys A Kenneth M. Y. Leung B Eric P. M. Grist A Mark Crane A The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.523.795 http://www.biosch.hku.hk/ecology/staffhp/kl/Time oyster.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.523.795 http://www.biosch.hku.hk/ecology/staffhp/kl/Time oyster.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.biosch.hku.hk/ecology/staffhp/kl/Time oyster.pdf text ftciteseerx 2016-01-08T10:15:57Z Developmental toxicity tests are often used for the hazard assessment of chemicals and environmental media. One of the most widely used is the oyster embryo larval test (OEL), in which the development of oyster larvae is arrested at a single fixed time (e.g. 24 or 48 h) of toxic exposure, and the proportion of normal larvae measured. However, a major problem with this conventional approach is the lack of information on temporal trends in development. In this study, Pacific oyster Crassostrea gigas embryos were exposed to nominal concentrations of copper (CuSO4) of <0.001 (control), 0.60, 1.25, 2.5, 5.0, 10.0 and 20.0 lg l1 (at 20 C, salinity 35 ‰ and pH 8.1). Three replicates from each group were arrested and examined every 8 h during 24–72 h of exposure, and the number of viable larvae developed to D-shape was determined. The results revealed that the number of viable D-shape larvae in the control increased rapidly and reached an optimum at 32 h, before declining gradually due to starvation. Analysis of covariance (ANCOVA) showed that larval developmental rates during 0–32 h were significantly inhibited by Cu at all concentrations. This paper demonstrates that arrest and measurement at different time periods are important and should be incorporated into the OEL test. This would maximise the sensitivity of the test in detecting developmental effects in spiked or environmental samples. Text Crassostrea gigas Pacific oyster Unknown Pacific |
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Developmental toxicity tests are often used for the hazard assessment of chemicals and environmental media. One of the most widely used is the oyster embryo larval test (OEL), in which the development of oyster larvae is arrested at a single fixed time (e.g. 24 or 48 h) of toxic exposure, and the proportion of normal larvae measured. However, a major problem with this conventional approach is the lack of information on temporal trends in development. In this study, Pacific oyster Crassostrea gigas embryos were exposed to nominal concentrations of copper (CuSO4) of <0.001 (control), 0.60, 1.25, 2.5, 5.0, 10.0 and 20.0 lg l1 (at 20 C, salinity 35 ‰ and pH 8.1). Three replicates from each group were arrested and examined every 8 h during 24–72 h of exposure, and the number of viable larvae developed to D-shape was determined. The results revealed that the number of viable D-shape larvae in the control increased rapidly and reached an optimum at 32 h, before declining gradually due to starvation. Analysis of covariance (ANCOVA) showed that larval developmental rates during 0–32 h were significantly inhibited by Cu at all concentrations. This paper demonstrates that arrest and measurement at different time periods are important and should be incorporated into the OEL test. This would maximise the sensitivity of the test in detecting developmental effects in spiked or environmental samples. |
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The Pennsylvania State University CiteSeerX Archives |
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Text |
author |
Mathew A. Worboys A Kenneth M. Y. Leung B Eric P. M. Grist A Mark Crane A |
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Mathew A. Worboys A Kenneth M. Y. Leung B Eric P. M. Grist A Mark Crane A Contributed papers Time should be considered in developmental ecotoxicity test |
author_facet |
Mathew A. Worboys A Kenneth M. Y. Leung B Eric P. M. Grist A Mark Crane A |
author_sort |
Mathew A. Worboys A |
title |
Contributed papers Time should be considered in developmental ecotoxicity test |
title_short |
Contributed papers Time should be considered in developmental ecotoxicity test |
title_full |
Contributed papers Time should be considered in developmental ecotoxicity test |
title_fullStr |
Contributed papers Time should be considered in developmental ecotoxicity test |
title_full_unstemmed |
Contributed papers Time should be considered in developmental ecotoxicity test |
title_sort |
contributed papers time should be considered in developmental ecotoxicity test |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.523.795 http://www.biosch.hku.hk/ecology/staffhp/kl/Time oyster.pdf |
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Pacific |
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Pacific |
genre |
Crassostrea gigas Pacific oyster |
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Crassostrea gigas Pacific oyster |
op_source |
http://www.biosch.hku.hk/ecology/staffhp/kl/Time oyster.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.523.795 http://www.biosch.hku.hk/ecology/staffhp/kl/Time oyster.pdf |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766394560614760448 |