We present evidence for a novel member of the hepadnavirus family that is endemic in wild arctic ground squirrels (Spermophylus parryi kennicotti) in Alaska. This virus, designated arctic squirrel hepatitis virus (ASHV), was initially detected in the livers of animals bearing large hepatic nodules b...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.522.8783 2023-05-15T14:52:37+02:00 The Pennsylvania State University CiteSeerX Archives 1995 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.522.8783 http://www.pasteur.fr/~tekaia/tekaia.JVI.1996.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.522.8783 http://www.pasteur.fr/~tekaia/tekaia.JVI.1996.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.pasteur.fr/~tekaia/tekaia.JVI.1996.pdf text 1995 ftciteseerx 2016-01-08T10:13:40Z We present evidence for a novel member of the hepadnavirus family that is endemic in wild arctic ground squirrels (Spermophylus parryi kennicotti) in Alaska. This virus, designated arctic squirrel hepatitis virus (ASHV), was initially detected in the livers of animals bearing large hepatic nodules by nucleic acid hybrid-ization with hepadnavirus probes and in plasma by cross-reactivity with antibodies to hepadnavirus surface and core antigens. The complete nucleotide sequence of the 3,302-bp-long ASHV genome was determined and compared with those of ground squirrel hepatitis virus (GSHV) and woodchuck hepatitis virus (WHV); all sequences were organized into four open reading frames, designated pre-C/C, pre-S/S, pol, and X. Despite roughly equivalent variability among the three rodent hepadnaviruses (around 16 % base and 19 % amino acid exchanges), ASHV appeared to be more closely related to GSHV than to WHV in phylogenetic analysis. Accordingly, preliminary studies of the pathology of ASHV infection suggested that ASHV may be a less efficient oncogenic agent than WHV. About one-third of aged animals maintained in captivity, including virus-infected as well as uninfected squirrels, developed large liver nodules, consisting of hepatocellular adenomas or carcinomas or nonmalignant lesions characterized by drastic microvesicular steatosis. ASHV-infected arctic ground squirrels may serve as a new model with which to analyze the contribution of hepad-navirus- and host-specific determinants to liver pathology and tumorigenesis. Text Arctic Alaska Unknown Arctic |
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ftciteseerx |
language |
English |
description |
We present evidence for a novel member of the hepadnavirus family that is endemic in wild arctic ground squirrels (Spermophylus parryi kennicotti) in Alaska. This virus, designated arctic squirrel hepatitis virus (ASHV), was initially detected in the livers of animals bearing large hepatic nodules by nucleic acid hybrid-ization with hepadnavirus probes and in plasma by cross-reactivity with antibodies to hepadnavirus surface and core antigens. The complete nucleotide sequence of the 3,302-bp-long ASHV genome was determined and compared with those of ground squirrel hepatitis virus (GSHV) and woodchuck hepatitis virus (WHV); all sequences were organized into four open reading frames, designated pre-C/C, pre-S/S, pol, and X. Despite roughly equivalent variability among the three rodent hepadnaviruses (around 16 % base and 19 % amino acid exchanges), ASHV appeared to be more closely related to GSHV than to WHV in phylogenetic analysis. Accordingly, preliminary studies of the pathology of ASHV infection suggested that ASHV may be a less efficient oncogenic agent than WHV. About one-third of aged animals maintained in captivity, including virus-infected as well as uninfected squirrels, developed large liver nodules, consisting of hepatocellular adenomas or carcinomas or nonmalignant lesions characterized by drastic microvesicular steatosis. ASHV-infected arctic ground squirrels may serve as a new model with which to analyze the contribution of hepad-navirus- and host-specific determinants to liver pathology and tumorigenesis. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
publishDate |
1995 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.522.8783 http://www.pasteur.fr/~tekaia/tekaia.JVI.1996.pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Alaska |
genre_facet |
Arctic Alaska |
op_source |
http://www.pasteur.fr/~tekaia/tekaia.JVI.1996.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.522.8783 http://www.pasteur.fr/~tekaia/tekaia.JVI.1996.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766323854022541312 |