Optimization of triploid induction by the use of 6-DMAP for the oyster Crassostrea gigas (Thunberg)

• Main Authors: A. Gerard, Y. Naciri, J. -m. Peignon, C. Ledu
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: Crassostrea gigas
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.518.9445; http://archimer.ifremer.fr/doc/1994/publication-3198.pdf

Abstracl. A nove! mcthod using 6-dimcthylaminopurinc (6-0MAP) has rcccntly bccn dcmonstrated to bc a good triploid inductor for bivalvc molluscs. Thc cffccts of 6-0MAP concentration, timing of trcatment aftcr fertilization. and treatment duration on survival and triploid induclion of Crassastrea gigas (Thunberg) embryos wcre examined. A.n influcnce of sperm concentration was also detected and discussed. Survival loO-stage was invcrscly re!ated to 6-0MAP concentration and the percentage of triploids was shown to bc 6-0MAP dose dependenl. Two optimal moments for treatment application were delcrmined. cor rcsponding to extrusions of the firsl and sceond polar bodies. Optimal treatment of 450 pmolll 6-0MAP bcginning 15 min after fertilization over a lOmin period yiclded mcan survival to O-stagc of 64 % with a mean of 85 % triploid production. As a comparison, a sample trcated with 1mg/l of cytoehalasin B (CB). 20 min aftcr fertilization over a 15 min pelriod, yieldcd a mean survival to O-stage of only 36 % with a mean of 95 % triploid production. The advantages of 6-0MAP ovcr CB arc clearly identified: this chemical is noll carcinogcnic, cheapcr than cytochalasin Band much casier to use as it is water soluble.