Population structure and stock identification of chum salmon from western Alaska determined with microsatellite and major histocompatibility complex variation. Pages 141-160. Pacific Salmon: ecology and management of western Alaska's populations

• Main Authors: Terry D. Beacham, Khai D. Le, John R. C
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Published: 2009
• Subjects: Chum salmon; microsatellites; population structure; stock identification; Fraser River; Keta; Pacific; Yukon; Yukon river; Alaska
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.514.3030; http://www.npafc.org/new/publications/Technical Report/TR5/page 31-33(Beacham).pdf

Stock identification of chum salmon (Oncorhynchus keta) migrating through particular locations on the high seas can be of scientific and management interest. Although allozyme-based methods of stock identification have proven useful in estimation of chum salmon stock composition in mixed-stock fisheries (Shaklee et al. 1999), and differentiation at allozyme loci occurs among chum salmon (Beacham et al. 1987; Seeb and Crane 1999), the level of discrimination available in some applications is not sufficient for fisheries management decisions. Variation in microsatellite loci has been applied in other species requiring discrimination among salmonid populations within watersheds (Small et al. 1998; Beacham and Wood 1999; Beacham et al. 2001), and has been shown to be useful in stock discrimination in chinook salmon (Banks et al. 2000). Variation at microsatellite loci has been particularly useful for population-specific estimates of stock composition of Fraser River chinook salmon (Beacham et al. 2003), and may work well for chum salmon. In the present study, we survey variation at 13 microsatellite loci in chum salmon, and evaluate the utility of using microsatellite variation for stock identification on a regional and local basis. This is accomplished by analysis of simulated mixtures containing chum salmon from different regions, and on a local basis by incorporation of specific chum salmon populations. Tissue samples were collected from adult chum salmon from populations in Japan, the Yukon River, southeast