A Novel lux Operon in the Cryptically Bioluminescent Fish Pathogen Vibrio salmonicida Is Associated with Virulence
The cold-water-fish pathogen Vibrio salmonicida expresses a functional bacterial luciferase but produces insufficient levels of its aliphatic-aldehyde substrate to be detectably luminous in culture. Our goals were to (i) better explain this cryptic bioluminescence phenotype through molecular charact...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.513.1179 2023-05-15T15:32:34+02:00 A Novel lux Operon in the Cryptically Bioluminescent Fish Pathogen Vibrio salmonicida Is Associated with Virulence The Pennsylvania State University CiteSeerX Archives 2006 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.513.1179 http://labs.medmicro.wisc.edu/mcfall_ruby_papers/pdf/2007/Nelson_Ruby_2007_ApplEnvironMicrobiol.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.513.1179 http://labs.medmicro.wisc.edu/mcfall_ruby_papers/pdf/2007/Nelson_Ruby_2007_ApplEnvironMicrobiol.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://labs.medmicro.wisc.edu/mcfall_ruby_papers/pdf/2007/Nelson_Ruby_2007_ApplEnvironMicrobiol.pdf text 2006 ftciteseerx 2016-01-08T09:44:33Z The cold-water-fish pathogen Vibrio salmonicida expresses a functional bacterial luciferase but produces insufficient levels of its aliphatic-aldehyde substrate to be detectably luminous in culture. Our goals were to (i) better explain this cryptic bioluminescence phenotype through molecular characterization of the lux operon and (ii) test whether the bioluminescence gene cluster is associated with virulence. Cloning and sequencing of the V. salmonicida lux operon revealed that homologs of all of the genes required for luminescence are present: luxAB (luciferase) and luxCDE (aliphatic-aldehyde synthesis). The arrangement and sequence of these struc-tural lux genes are conserved compared to those in related species of luminous bacteria. However, V. salmo-nicida strains have a novel arrangement and number of homologs of the luxR and luxI quorum-sensing regulatory genes. Reverse transcriptase PCR analysis suggests that this novel arrangement of quorum-sensing genes generates antisense transcripts that may be responsible for the reduced production of bioluminescence. In addition, infection with a strain in which the luxA gene was mutated resulted in a marked delay in mortality among Atlantic salmon relative to infection with the wild-type parent in single-strain challenge experiments. In mixed-strain competition between the luxA mutant and the wild type, the mutant was attenuated up to 50-fold. It remains unclear whether the attenuation results from a direct loss of luciferase or a polar Text Atlantic salmon Unknown |
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English |
description |
The cold-water-fish pathogen Vibrio salmonicida expresses a functional bacterial luciferase but produces insufficient levels of its aliphatic-aldehyde substrate to be detectably luminous in culture. Our goals were to (i) better explain this cryptic bioluminescence phenotype through molecular characterization of the lux operon and (ii) test whether the bioluminescence gene cluster is associated with virulence. Cloning and sequencing of the V. salmonicida lux operon revealed that homologs of all of the genes required for luminescence are present: luxAB (luciferase) and luxCDE (aliphatic-aldehyde synthesis). The arrangement and sequence of these struc-tural lux genes are conserved compared to those in related species of luminous bacteria. However, V. salmo-nicida strains have a novel arrangement and number of homologs of the luxR and luxI quorum-sensing regulatory genes. Reverse transcriptase PCR analysis suggests that this novel arrangement of quorum-sensing genes generates antisense transcripts that may be responsible for the reduced production of bioluminescence. In addition, infection with a strain in which the luxA gene was mutated resulted in a marked delay in mortality among Atlantic salmon relative to infection with the wild-type parent in single-strain challenge experiments. In mixed-strain competition between the luxA mutant and the wild type, the mutant was attenuated up to 50-fold. It remains unclear whether the attenuation results from a direct loss of luciferase or a polar |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
title |
A Novel lux Operon in the Cryptically Bioluminescent Fish Pathogen Vibrio salmonicida Is Associated with Virulence |
spellingShingle |
A Novel lux Operon in the Cryptically Bioluminescent Fish Pathogen Vibrio salmonicida Is Associated with Virulence |
title_short |
A Novel lux Operon in the Cryptically Bioluminescent Fish Pathogen Vibrio salmonicida Is Associated with Virulence |
title_full |
A Novel lux Operon in the Cryptically Bioluminescent Fish Pathogen Vibrio salmonicida Is Associated with Virulence |
title_fullStr |
A Novel lux Operon in the Cryptically Bioluminescent Fish Pathogen Vibrio salmonicida Is Associated with Virulence |
title_full_unstemmed |
A Novel lux Operon in the Cryptically Bioluminescent Fish Pathogen Vibrio salmonicida Is Associated with Virulence |
title_sort |
novel lux operon in the cryptically bioluminescent fish pathogen vibrio salmonicida is associated with virulence |
publishDate |
2006 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.513.1179 http://labs.medmicro.wisc.edu/mcfall_ruby_papers/pdf/2007/Nelson_Ruby_2007_ApplEnvironMicrobiol.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
http://labs.medmicro.wisc.edu/mcfall_ruby_papers/pdf/2007/Nelson_Ruby_2007_ApplEnvironMicrobiol.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.513.1179 http://labs.medmicro.wisc.edu/mcfall_ruby_papers/pdf/2007/Nelson_Ruby_2007_ApplEnvironMicrobiol.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766363065738067968 |