Molecular characterization of a cDNA encoding caffeoyl-coenzyme A 3-O-methyltransferase
Abstract. A cDNA clone encoding S-adenosyl-L-methionine: trans-caffeoyl-CoA 3-O-methyl-transferase (EC 2·1·1·104; CCoAOMT) from Stellana longipes Goldie (long-stalked chick-weed) was isolated and studied. Structural analysis of both the nucleotide sequence and the predicted amino acid sequence sugge...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.510.8626 2023-05-15T18:27:18+02:00 Molecular characterization of a cDNA encoding caffeoyl-coenzyme A 3-O-methyltransferase The Pennsylvania State University CiteSeerX Archives 1996 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.510.8626 http://www.ias.ac.in/jarch/jbiosci/22/161-175.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.510.8626 http://www.ias.ac.in/jarch/jbiosci/22/161-175.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.ias.ac.in/jarch/jbiosci/22/161-175.pdf caffeic acid 3-O-methyltransferase cDNA phenylpropanoid metabolism Stellaria longipes text 1996 ftciteseerx 2016-01-08T09:38:30Z Abstract. A cDNA clone encoding S-adenosyl-L-methionine: trans-caffeoyl-CoA 3-O-methyl-transferase (EC 2·1·1·104; CCoAOMT) from Stellana longipes Goldie (long-stalked chick-weed) was isolated and studied. Structural analysis of both the nucleotide sequence and the predicted amino acid sequence suggests that our cloned sequence encoded a CCoAOMT enzyme of Stellaria longipes, which shared overall structural similarity with other plant CCoAOMTs but exhibited certain distinct characteristics. Southern blot hybridization and cloning analyses indicating a small CCoAOMT gene family in the Stellana longipes genome and the absence of introns in the coding region of the cDNA- corresponding gene. Sequence variations in the coding region were found among three genotypes from geographically isolated populations. Higher levels of CCoAOMT mRNA were detected in stems and leaves than in roots. The cDNA-encoded protein expressed in Eschendia coli was shown to utilize caffeoyl-CoA, but not caffeic acid or 5-hydroxy ferulic acid, as its substrate. Text Stellaria longipes Unknown Goldie ENVELOPE(165.900,165.900,-82.633,-82.633) |
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Unknown |
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ftciteseerx |
language |
English |
topic |
caffeic acid 3-O-methyltransferase cDNA phenylpropanoid metabolism Stellaria longipes |
spellingShingle |
caffeic acid 3-O-methyltransferase cDNA phenylpropanoid metabolism Stellaria longipes Molecular characterization of a cDNA encoding caffeoyl-coenzyme A 3-O-methyltransferase |
topic_facet |
caffeic acid 3-O-methyltransferase cDNA phenylpropanoid metabolism Stellaria longipes |
description |
Abstract. A cDNA clone encoding S-adenosyl-L-methionine: trans-caffeoyl-CoA 3-O-methyl-transferase (EC 2·1·1·104; CCoAOMT) from Stellana longipes Goldie (long-stalked chick-weed) was isolated and studied. Structural analysis of both the nucleotide sequence and the predicted amino acid sequence suggests that our cloned sequence encoded a CCoAOMT enzyme of Stellaria longipes, which shared overall structural similarity with other plant CCoAOMTs but exhibited certain distinct characteristics. Southern blot hybridization and cloning analyses indicating a small CCoAOMT gene family in the Stellana longipes genome and the absence of introns in the coding region of the cDNA- corresponding gene. Sequence variations in the coding region were found among three genotypes from geographically isolated populations. Higher levels of CCoAOMT mRNA were detected in stems and leaves than in roots. The cDNA-encoded protein expressed in Eschendia coli was shown to utilize caffeoyl-CoA, but not caffeic acid or 5-hydroxy ferulic acid, as its substrate. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
title |
Molecular characterization of a cDNA encoding caffeoyl-coenzyme A 3-O-methyltransferase |
title_short |
Molecular characterization of a cDNA encoding caffeoyl-coenzyme A 3-O-methyltransferase |
title_full |
Molecular characterization of a cDNA encoding caffeoyl-coenzyme A 3-O-methyltransferase |
title_fullStr |
Molecular characterization of a cDNA encoding caffeoyl-coenzyme A 3-O-methyltransferase |
title_full_unstemmed |
Molecular characterization of a cDNA encoding caffeoyl-coenzyme A 3-O-methyltransferase |
title_sort |
molecular characterization of a cdna encoding caffeoyl-coenzyme a 3-o-methyltransferase |
publishDate |
1996 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.510.8626 http://www.ias.ac.in/jarch/jbiosci/22/161-175.pdf |
long_lat |
ENVELOPE(165.900,165.900,-82.633,-82.633) |
geographic |
Goldie |
geographic_facet |
Goldie |
genre |
Stellaria longipes |
genre_facet |
Stellaria longipes |
op_source |
http://www.ias.ac.in/jarch/jbiosci/22/161-175.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.510.8626 http://www.ias.ac.in/jarch/jbiosci/22/161-175.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766209355376492544 |