Summary
Although urea transport is receiving increased attention in mammalian systems, very little is known about urea transport in fish tissues. This study examined mechanisms of urea transport in red blood cells and hepatocytes from the lesser spotted dogfish (Scyliorhinus canicula), Atlantic stingray (Da...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.500.7999 2023-05-15T18:41:14+02:00 Summary Patrick J. Walsh Chris M. Wood Steve F. Perry The Pennsylvania State University CiteSeerX Archives 1994 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.500.7999 http://jeb.biologists.org/content/193/1/321.full.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.500.7999 http://jeb.biologists.org/content/193/1/321.full.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://jeb.biologists.org/content/193/1/321.full.pdf Key words urea transport red blood cells hepatocytes Opsanus beta Opsanus tau Dasyatis sabina text 1994 ftciteseerx 2016-01-08T09:07:36Z Although urea transport is receiving increased attention in mammalian systems, very little is known about urea transport in fish tissues. This study examined mechanisms of urea transport in red blood cells and hepatocytes from the lesser spotted dogfish (Scyliorhinus canicula), Atlantic stingray (Dasyatis sabina), turbot (Scopthalmus maximus), redfish (Scianops ocellatus), gulf toadfish (Opsanus beta) and oyster toadfish (Opsanus tau). Urea appeared to be passively distributed in both tissues (i.e. there was no difference between plasma and tissue urea concentrations). Additionally, a number of in vitro experiments examining [14C]urea flux were performed. In red blood cells from all species except redfish, urea transport occurred via simple passive diffusion, but redfish red blood cells showed a small (25 %) phloretin-sensitive uptake component. In hepatocytes of the two elasmobranch species (dogfish and stingray), urea efflux was also by simple passive diffusion. However, urea efflux in toadfish (both O. beta and O. tau) hepatocytes exhibited a marked phloretin-sensitivity, and O. beta hepatocytes were used in further experiments with other inhibitors and treatments. Urea transport in O. beta had a relatively high specificity for urea compared with the urea analogues acetamide Text Turbot Unknown Canicula ENVELOPE(-58.515,-58.515,-63.717,-63.717) |
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English |
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Key words urea transport red blood cells hepatocytes Opsanus beta Opsanus tau Dasyatis sabina |
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Key words urea transport red blood cells hepatocytes Opsanus beta Opsanus tau Dasyatis sabina Patrick J. Walsh Chris M. Wood Steve F. Perry Summary |
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Key words urea transport red blood cells hepatocytes Opsanus beta Opsanus tau Dasyatis sabina |
description |
Although urea transport is receiving increased attention in mammalian systems, very little is known about urea transport in fish tissues. This study examined mechanisms of urea transport in red blood cells and hepatocytes from the lesser spotted dogfish (Scyliorhinus canicula), Atlantic stingray (Dasyatis sabina), turbot (Scopthalmus maximus), redfish (Scianops ocellatus), gulf toadfish (Opsanus beta) and oyster toadfish (Opsanus tau). Urea appeared to be passively distributed in both tissues (i.e. there was no difference between plasma and tissue urea concentrations). Additionally, a number of in vitro experiments examining [14C]urea flux were performed. In red blood cells from all species except redfish, urea transport occurred via simple passive diffusion, but redfish red blood cells showed a small (25 %) phloretin-sensitive uptake component. In hepatocytes of the two elasmobranch species (dogfish and stingray), urea efflux was also by simple passive diffusion. However, urea efflux in toadfish (both O. beta and O. tau) hepatocytes exhibited a marked phloretin-sensitivity, and O. beta hepatocytes were used in further experiments with other inhibitors and treatments. Urea transport in O. beta had a relatively high specificity for urea compared with the urea analogues acetamide |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Patrick J. Walsh Chris M. Wood Steve F. Perry |
author_facet |
Patrick J. Walsh Chris M. Wood Steve F. Perry |
author_sort |
Patrick J. Walsh |
title |
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title_full |
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publishDate |
1994 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.500.7999 http://jeb.biologists.org/content/193/1/321.full.pdf |
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ENVELOPE(-58.515,-58.515,-63.717,-63.717) |
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Canicula |
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Turbot |
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Turbot |
op_source |
http://jeb.biologists.org/content/193/1/321.full.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.500.7999 http://jeb.biologists.org/content/193/1/321.full.pdf |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766230726065258496 |