Bacterial activity at –2 to –20◦C in arctic wintertime sea ice
Arctic wintertime sea-ice cores, characterized by a temperature gradient of 2 to 20°C, were investigated to better understand constraints on bacterial abundance, activity, and diversity at subzero temperatures. With the fluorescent stains 4,6-diamidino-2-phenylindole 2HCl (DAPI) (for DNA) and 5-cyan...
Main Authors: | , , |
---|---|
Other Authors: | |
Format: | Text |
Language: | English |
Subjects: | |
Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.499.3191 http://www2.gi.alaska.edu/~eicken/he_publ/04JED.pdf |
id |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.499.3191 |
---|---|
record_format |
openpolar |
spelling |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.499.3191 2023-05-15T14:59:24+02:00 Bacterial activity at –2 to –20◦C in arctic wintertime sea ice Karen Junge Hajo Eicken Jody W. Deming The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.499.3191 http://www2.gi.alaska.edu/~eicken/he_publ/04JED.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.499.3191 http://www2.gi.alaska.edu/~eicken/he_publ/04JED.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www2.gi.alaska.edu/~eicken/he_publ/04JED.pdf text ftciteseerx 2016-01-08T08:57:24Z Arctic wintertime sea-ice cores, characterized by a temperature gradient of 2 to 20°C, were investigated to better understand constraints on bacterial abundance, activity, and diversity at subzero temperatures. With the fluorescent stains 4,6-diamidino-2-phenylindole 2HCl (DAPI) (for DNA) and 5-cyano-2,3-ditoyl tetrazo-lium chloride (CTC) (for O2-based respiration), the abundances of total, particle-associated (>3-m), free-living, and actively respiring bacteria were determined for ice-core samples melted at their in situ temperatures (2 to20°C) and at the corresponding salinities of their brine inclusions (38 to 209 ppt). Fluorescence in situ hybridization was applied to determine the proportions of Bacteria, Cytophaga-Flavobacteria-Bacteroides (CFB), and Archaea. Microtome-prepared ice sections also were examined microscopically under in situ conditions to evaluate bacterial abundance (by DAPI staining) and particle associations within the brine-inclusion network of the ice. For both melted and intact ice sections, more than 50 % of cells were found to be associated with particles or surfaces (sediment grains, detritus, and ice-crystal boundaries). CTC-active bacteria (0.5 to 4 % of the total) and cells detectable by rRNA probes (18 to 86 % of the total) were found in all ice samples, including the coldest (20°C), where virtually all active cells were particle associated. The percentage of active bacteria associated with particles increased with decreasing temperature, as did the percentages of CFB (16 to 82 % of Bacteria) and Archaea (0.0 to 3.4 % of total cells). These results, combined with correlation analyses between Text Arctic ice core Sea ice Unknown Arctic Lium ENVELOPE(18.897,18.897,68.991,68.991) |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftciteseerx |
language |
English |
description |
Arctic wintertime sea-ice cores, characterized by a temperature gradient of 2 to 20°C, were investigated to better understand constraints on bacterial abundance, activity, and diversity at subzero temperatures. With the fluorescent stains 4,6-diamidino-2-phenylindole 2HCl (DAPI) (for DNA) and 5-cyano-2,3-ditoyl tetrazo-lium chloride (CTC) (for O2-based respiration), the abundances of total, particle-associated (>3-m), free-living, and actively respiring bacteria were determined for ice-core samples melted at their in situ temperatures (2 to20°C) and at the corresponding salinities of their brine inclusions (38 to 209 ppt). Fluorescence in situ hybridization was applied to determine the proportions of Bacteria, Cytophaga-Flavobacteria-Bacteroides (CFB), and Archaea. Microtome-prepared ice sections also were examined microscopically under in situ conditions to evaluate bacterial abundance (by DAPI staining) and particle associations within the brine-inclusion network of the ice. For both melted and intact ice sections, more than 50 % of cells were found to be associated with particles or surfaces (sediment grains, detritus, and ice-crystal boundaries). CTC-active bacteria (0.5 to 4 % of the total) and cells detectable by rRNA probes (18 to 86 % of the total) were found in all ice samples, including the coldest (20°C), where virtually all active cells were particle associated. The percentage of active bacteria associated with particles increased with decreasing temperature, as did the percentages of CFB (16 to 82 % of Bacteria) and Archaea (0.0 to 3.4 % of total cells). These results, combined with correlation analyses between |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Karen Junge Hajo Eicken Jody W. Deming |
spellingShingle |
Karen Junge Hajo Eicken Jody W. Deming Bacterial activity at –2 to –20◦C in arctic wintertime sea ice |
author_facet |
Karen Junge Hajo Eicken Jody W. Deming |
author_sort |
Karen Junge |
title |
Bacterial activity at –2 to –20◦C in arctic wintertime sea ice |
title_short |
Bacterial activity at –2 to –20◦C in arctic wintertime sea ice |
title_full |
Bacterial activity at –2 to –20◦C in arctic wintertime sea ice |
title_fullStr |
Bacterial activity at –2 to –20◦C in arctic wintertime sea ice |
title_full_unstemmed |
Bacterial activity at –2 to –20◦C in arctic wintertime sea ice |
title_sort |
bacterial activity at –2 to –20◦c in arctic wintertime sea ice |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.499.3191 http://www2.gi.alaska.edu/~eicken/he_publ/04JED.pdf |
long_lat |
ENVELOPE(18.897,18.897,68.991,68.991) |
geographic |
Arctic Lium |
geographic_facet |
Arctic Lium |
genre |
Arctic ice core Sea ice |
genre_facet |
Arctic ice core Sea ice |
op_source |
http://www2.gi.alaska.edu/~eicken/he_publ/04JED.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.499.3191 http://www2.gi.alaska.edu/~eicken/he_publ/04JED.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766331512146362368 |