Molecular Characterization of the Rocky Mountain Elk (Cervus elaphus nelsoni) PRNP Putative Promoter
Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) affecting deer (Odocoileus spp.), moose (Alces alces), and Rocky Mountain elk (Cervus elaphus nelsoni). Leucine homozygosity at elk PRNP codon 132 has been associated with reduced CWD susceptibility. However, naturally...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.497.804 2023-05-15T13:13:22+02:00 Molecular Characterization of the Rocky Mountain Elk (Cervus elaphus nelsoni) PRNP Putative Promoter Christopher M. Seabury Clare A. Gill Joe W. Templeton Joseph B. Dyar James N. Derr L. Adelson Elaine Owens Donald S. Davis Duane C. Kraemer James E. Womack The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.497.804 http://jhered.oxfordjournals.org/content/early/2007/11/21/jhered.esm091.full.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.497.804 http://jhered.oxfordjournals.org/content/early/2007/11/21/jhered.esm091.full.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://jhered.oxfordjournals.org/content/early/2007/11/21/jhered.esm091.full.pdf text ftciteseerx 2016-01-08T08:52:34Z Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) affecting deer (Odocoileus spp.), moose (Alces alces), and Rocky Mountain elk (Cervus elaphus nelsoni). Leucine homozygosity at elk PRNP codon 132 has been associated with reduced CWD susceptibility. However, naturally acquired CWD has been detected in elk possessing the 132 Leu/Leu genotype. Recent human and bovine studies indicate that PRNP regulatory polymorphisms may also influence TSE occurrence. Therefore, we generated sequences for the elk PRNP putative promoter (2.2 kb), exon 1 (predicted; 54 bp), intron 1 (predicted; 193 bp), and exon 3 (771 bp). Promoter prediction analysis using CpGProD yielded a single elk PRNP promoter that was homologous to regions of known promoter activity in cow and sheep. Molecular interrogation of the elk PRNP putative promoter revealed 32 diallelic single-nucleotide polymorphisms (SNPs). No variation was detected within the predicted exon 1 or intron 1 sequences. Evaluation of elk PRNP exon 3 revealed 3 SNPs (63Y, 312R, 394W/Met/Leu). Bayesian haplotype reconstruction resulted in 3 elk PRNP haplotypes, with complete linkage disequilibrium observed between all PRNP putative promoter SNPs and codon 132. The results of this study provide the initial genomic foundation for future comparative and haplotype-based elk PRNP studies. Transmissible spongiform encephalopathies (TSEs), or prion Text Alces alces Unknown |
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description |
Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) affecting deer (Odocoileus spp.), moose (Alces alces), and Rocky Mountain elk (Cervus elaphus nelsoni). Leucine homozygosity at elk PRNP codon 132 has been associated with reduced CWD susceptibility. However, naturally acquired CWD has been detected in elk possessing the 132 Leu/Leu genotype. Recent human and bovine studies indicate that PRNP regulatory polymorphisms may also influence TSE occurrence. Therefore, we generated sequences for the elk PRNP putative promoter (2.2 kb), exon 1 (predicted; 54 bp), intron 1 (predicted; 193 bp), and exon 3 (771 bp). Promoter prediction analysis using CpGProD yielded a single elk PRNP promoter that was homologous to regions of known promoter activity in cow and sheep. Molecular interrogation of the elk PRNP putative promoter revealed 32 diallelic single-nucleotide polymorphisms (SNPs). No variation was detected within the predicted exon 1 or intron 1 sequences. Evaluation of elk PRNP exon 3 revealed 3 SNPs (63Y, 312R, 394W/Met/Leu). Bayesian haplotype reconstruction resulted in 3 elk PRNP haplotypes, with complete linkage disequilibrium observed between all PRNP putative promoter SNPs and codon 132. The results of this study provide the initial genomic foundation for future comparative and haplotype-based elk PRNP studies. Transmissible spongiform encephalopathies (TSEs), or prion |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Christopher M. Seabury Clare A. Gill Joe W. Templeton Joseph B. Dyar James N. Derr L. Adelson Elaine Owens Donald S. Davis Duane C. Kraemer James E. Womack |
spellingShingle |
Christopher M. Seabury Clare A. Gill Joe W. Templeton Joseph B. Dyar James N. Derr L. Adelson Elaine Owens Donald S. Davis Duane C. Kraemer James E. Womack Molecular Characterization of the Rocky Mountain Elk (Cervus elaphus nelsoni) PRNP Putative Promoter |
author_facet |
Christopher M. Seabury Clare A. Gill Joe W. Templeton Joseph B. Dyar James N. Derr L. Adelson Elaine Owens Donald S. Davis Duane C. Kraemer James E. Womack |
author_sort |
Christopher M. Seabury |
title |
Molecular Characterization of the Rocky Mountain Elk (Cervus elaphus nelsoni) PRNP Putative Promoter |
title_short |
Molecular Characterization of the Rocky Mountain Elk (Cervus elaphus nelsoni) PRNP Putative Promoter |
title_full |
Molecular Characterization of the Rocky Mountain Elk (Cervus elaphus nelsoni) PRNP Putative Promoter |
title_fullStr |
Molecular Characterization of the Rocky Mountain Elk (Cervus elaphus nelsoni) PRNP Putative Promoter |
title_full_unstemmed |
Molecular Characterization of the Rocky Mountain Elk (Cervus elaphus nelsoni) PRNP Putative Promoter |
title_sort |
molecular characterization of the rocky mountain elk (cervus elaphus nelsoni) prnp putative promoter |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.497.804 http://jhered.oxfordjournals.org/content/early/2007/11/21/jhered.esm091.full.pdf |
genre |
Alces alces |
genre_facet |
Alces alces |
op_source |
http://jhered.oxfordjournals.org/content/early/2007/11/21/jhered.esm091.full.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.497.804 http://jhered.oxfordjournals.org/content/early/2007/11/21/jhered.esm091.full.pdf |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766257932789350400 |