Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides
Abstract The insulin-like growth factor type 1 receptor (IGF-IR) belongs to the tyrosine kinase (TK) receptor family. Besides being mitogenic, IGF-IR plays a crucial role in cell survival, transformation and maintenance of the malignant phenotype. In this study, we cloned the cDNA from the hypothala...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.489.9654 2023-05-15T18:41:11+02:00 Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides Yue-mei Kuang Wen-sheng Li Hao-ran Lin The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.489.9654 http://www.abbs.info/pdf/37-5/6-327-334-05005.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.489.9654 http://www.abbs.info/pdf/37-5/6-327-334-05005.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.abbs.info/pdf/37-5/6-327-334-05005.pdf text ftciteseerx 2016-01-08T08:24:42Z Abstract The insulin-like growth factor type 1 receptor (IGF-IR) belongs to the tyrosine kinase (TK) receptor family. Besides being mitogenic, IGF-IR plays a crucial role in cell survival, transformation and maintenance of the malignant phenotype. In this study, we cloned the cDNA from the hypothalamus of the orange-spotted grouper (Epinephelus coioides) using reverse transcription PCR (RT-PCR) and the rapid amplification of cDNA ends (RACE) method. The deduced amino acid sequence showed that the receptor comprises 1413 amino acid residues. It contains cysteine-rich domains in its α-subunit, and a conserved transmembrane domain and TK domains in its β-subunit. Comparison of the amino acid sequence with those of other species showed that the grouper IGF-IR shares 90.2%, 89.6%, 71.9 % and 72 % similarity with the IGF-IR of the Japanese flounder, turbot, zebrafish-a and zebrafish-b, respectively. When compared with its mammalian homologue, grouper IGF-IR contains a large insertion at its C-terminus. Phylogenetic analysis has revealed that the grouper IGF-IR belongs to the b-type IGF-IRs and has a higher similarity with flounder and turbot IGF-IR, and a lower similarity (<70%) with human, mouse and avian IGF-IR. Grouper IGF-IR transcripts were detected in the brain, peripheral tissues, embryos and early development larvae by semi-quantitative RT-PCR assay. It was observed that IGF-IR mRNA expression was greater in the brain than in peripheral tissues. The level of IGF-IR mRNA expression was much higher in retina, gonad, skeletal Text Turbot Unknown |
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Abstract The insulin-like growth factor type 1 receptor (IGF-IR) belongs to the tyrosine kinase (TK) receptor family. Besides being mitogenic, IGF-IR plays a crucial role in cell survival, transformation and maintenance of the malignant phenotype. In this study, we cloned the cDNA from the hypothalamus of the orange-spotted grouper (Epinephelus coioides) using reverse transcription PCR (RT-PCR) and the rapid amplification of cDNA ends (RACE) method. The deduced amino acid sequence showed that the receptor comprises 1413 amino acid residues. It contains cysteine-rich domains in its α-subunit, and a conserved transmembrane domain and TK domains in its β-subunit. Comparison of the amino acid sequence with those of other species showed that the grouper IGF-IR shares 90.2%, 89.6%, 71.9 % and 72 % similarity with the IGF-IR of the Japanese flounder, turbot, zebrafish-a and zebrafish-b, respectively. When compared with its mammalian homologue, grouper IGF-IR contains a large insertion at its C-terminus. Phylogenetic analysis has revealed that the grouper IGF-IR belongs to the b-type IGF-IRs and has a higher similarity with flounder and turbot IGF-IR, and a lower similarity (<70%) with human, mouse and avian IGF-IR. Grouper IGF-IR transcripts were detected in the brain, peripheral tissues, embryos and early development larvae by semi-quantitative RT-PCR assay. It was observed that IGF-IR mRNA expression was greater in the brain than in peripheral tissues. The level of IGF-IR mRNA expression was much higher in retina, gonad, skeletal |
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The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Yue-mei Kuang Wen-sheng Li Hao-ran Lin |
spellingShingle |
Yue-mei Kuang Wen-sheng Li Hao-ran Lin Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides |
author_facet |
Yue-mei Kuang Wen-sheng Li Hao-ran Lin |
author_sort |
Yue-mei Kuang |
title |
Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides |
title_short |
Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides |
title_full |
Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides |
title_fullStr |
Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides |
title_full_unstemmed |
Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides |
title_sort |
molecular cloning and mrna profile of insulin-like growth factor type 1 receptor in orange-spotted grouper, epinephelus coioides |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.489.9654 http://www.abbs.info/pdf/37-5/6-327-334-05005.pdf |
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http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.489.9654 http://www.abbs.info/pdf/37-5/6-327-334-05005.pdf |
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