Coupling Constants in C”O- and 13CO-Labeled Species?
ABSTRACT: W e have obtained the oxygen-17 nuclear magnetic resonance ( N M R) spectra of a variety of CI70-labeled heme proteins, including sperm whale (Physeter cutodon) myoglobin, two synthetic sperm whale myoglobin mutants (His E7- Val E7; His E7- Phe E7), adult human hemoglobin, rabbit (Or-yctol...
Main Authors: | , , , , , |
---|---|
Other Authors: | |
Format: | Text |
Language: | English |
Published: |
1990
|
Subjects: | |
Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.467.9871 |
id |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.467.9871 |
---|---|
record_format |
openpolar |
spelling |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.467.9871 2023-05-15T18:26:40+02:00 Coupling Constants in C”O- and 13CO-Labeled Species? Ki Deok Park Kermin Guo Foluso Adebodun Mark L. Chiu Stephen G. Sligar Eric Oldfield The Pennsylvania State University CiteSeerX Archives 1990 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.467.9871 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.467.9871 Metadata may be used without restrictions as long as the oai identifier remains attached to it. https://chad.scs.uiuc.edu/docs/PDF/1201228223_145.pdf text 1990 ftciteseerx 2016-01-08T07:02:58Z ABSTRACT: W e have obtained the oxygen-17 nuclear magnetic resonance ( N M R) spectra of a variety of CI70-labeled heme proteins, including sperm whale (Physeter cutodon) myoglobin, two synthetic sperm whale myoglobin mutants (His E7- Val E7; His E7- Phe E7), adult human hemoglobin, rabbit (Or-yctolagus cuniculus) hemoglobin, horseradish (Cochlearia armoracia) peroxidase (E.C. 1.1 1.1.7) isoenzymes A and C, and Culduriomyces fumago chloroperoxidase (E.C. 1.1 1.1. lo), in some cases as a function of pH, and have determined their isotropic I7O N M R chemical shifts, ai, and spin-lattice relaxation times, T I. W e have also obtained similar results on a picket fence prophyrin, [ 5,10,15,20-tetrakis(a,a,a,a-pivalamido-phenyl)porphyrinato]iron(lI) ( l-MeIm)CO, both in solution and in the solid state. Our results show an excellent correlation between the infrared C- 0 vibrational frequencies, v(C-0), and ai, between u(C-0) and the I7O nuclear quadrupole coupling constant ($qQ/h, derived from T I) , and as expected between $qQ/h and ai. Taken together with the work of others on the I3C N M R of I3CO-labeled proteins, where we find an excellent correlation between Bi(13C) and u(Fe-C), our results suggest that I R and N M R measurements reflect the same interaction, which is thought to be primarily the degree of n-back-bonding from Fe d to Text Sperm whale Unknown |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftciteseerx |
language |
English |
description |
ABSTRACT: W e have obtained the oxygen-17 nuclear magnetic resonance ( N M R) spectra of a variety of CI70-labeled heme proteins, including sperm whale (Physeter cutodon) myoglobin, two synthetic sperm whale myoglobin mutants (His E7- Val E7; His E7- Phe E7), adult human hemoglobin, rabbit (Or-yctolagus cuniculus) hemoglobin, horseradish (Cochlearia armoracia) peroxidase (E.C. 1.1 1.1.7) isoenzymes A and C, and Culduriomyces fumago chloroperoxidase (E.C. 1.1 1.1. lo), in some cases as a function of pH, and have determined their isotropic I7O N M R chemical shifts, ai, and spin-lattice relaxation times, T I. W e have also obtained similar results on a picket fence prophyrin, [ 5,10,15,20-tetrakis(a,a,a,a-pivalamido-phenyl)porphyrinato]iron(lI) ( l-MeIm)CO, both in solution and in the solid state. Our results show an excellent correlation between the infrared C- 0 vibrational frequencies, v(C-0), and ai, between u(C-0) and the I7O nuclear quadrupole coupling constant ($qQ/h, derived from T I) , and as expected between $qQ/h and ai. Taken together with the work of others on the I3C N M R of I3CO-labeled proteins, where we find an excellent correlation between Bi(13C) and u(Fe-C), our results suggest that I R and N M R measurements reflect the same interaction, which is thought to be primarily the degree of n-back-bonding from Fe d to |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Ki Deok Park Kermin Guo Foluso Adebodun Mark L. Chiu Stephen G. Sligar Eric Oldfield |
spellingShingle |
Ki Deok Park Kermin Guo Foluso Adebodun Mark L. Chiu Stephen G. Sligar Eric Oldfield Coupling Constants in C”O- and 13CO-Labeled Species? |
author_facet |
Ki Deok Park Kermin Guo Foluso Adebodun Mark L. Chiu Stephen G. Sligar Eric Oldfield |
author_sort |
Ki Deok Park |
title |
Coupling Constants in C”O- and 13CO-Labeled Species? |
title_short |
Coupling Constants in C”O- and 13CO-Labeled Species? |
title_full |
Coupling Constants in C”O- and 13CO-Labeled Species? |
title_fullStr |
Coupling Constants in C”O- and 13CO-Labeled Species? |
title_full_unstemmed |
Coupling Constants in C”O- and 13CO-Labeled Species? |
title_sort |
coupling constants in c”o- and 13co-labeled species? |
publishDate |
1990 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.467.9871 |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
https://chad.scs.uiuc.edu/docs/PDF/1201228223_145.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.467.9871 |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766208642624782336 |