Microscopy

• Main Author: Pigment Data
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Published: 2011
• Subjects: Antarctic; Antarctic Peninsula; Antarc*
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.467.8636; http://pal.lternet.edu/docs/bibliography/Public/358lterc.pdf

a b s t r a c t Presented is an evaluation of the application of CHEMTAX (CHEMical TAXonomy) to Antarctic coastal pigments collected along the western Antarctic Peninsula (wAP). Overall analytical error is o20 % for all pigments involved in the analysis. CHEMTAX was stable within a range of input pigment ratios; data were analyzed in three bins based on light depths, with each year’s data run independently. Results were validated by comparison to those from CHEMTAX methods that included randomized error, feedback loops and additional diagnostic pigments. Blooms during mid-summer (chlorophyll a concentrations 45 mg L!1) were dominated primarily by either diatoms or cryptomonads. Mixed flagellates can also be abundant and Pheaocystis spp. and prasinophytes are frequently present in low concentrations. Comparison with microscopy shows CHEMTAX to give superior results in identifying Pheaocystis spp. with favorable results for other groups. This analysis shows CHEMTAX to be a reliable and stable tool for providing estimations of the main phytoplankton taxa in wAP waters based on long-term data collected during a 12-year time series. & 2011 Elsevier Ltd. All rights reserved. 1.