Methanogen Communities in a Drained Bog: Effect of Ash Fertilization
Forestry practises such has drainage have been shown to decrease emissions of the greenhouse gas methane (CH4) from peatlands. The aim of the study was to examine the methanogen populations in a drained bog in northern Finland, and to assess the possible effect of ash fertiliza-tion on potential met...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.464.6823 2023-05-15T17:42:35+02:00 Methanogen Communities in a Drained Bog: Effect of Ash Fertilization P. E. Gal H. Juottonen H. Fritze The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.464.6823 http://132.203.57.253/warwickvincent/PDFfiles/pierreMicrobialecol2005.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.464.6823 http://132.203.57.253/warwickvincent/PDFfiles/pierreMicrobialecol2005.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://132.203.57.253/warwickvincent/PDFfiles/pierreMicrobialecol2005.pdf text ftciteseerx 2016-01-08T06:50:16Z Forestry practises such has drainage have been shown to decrease emissions of the greenhouse gas methane (CH4) from peatlands. The aim of the study was to examine the methanogen populations in a drained bog in northern Finland, and to assess the possible effect of ash fertiliza-tion on potential methane production and methanogen communities. Peat samples were collected from control and ash fertilized (15,000 kg/ha) plots 5 years after ash application, and potential CH4 production was mea-sured. The methanogen community structure was stud-ied by DNA isolation, PCR amplification of the methyl coenzyme-M reductase (mcr) gene, denaturing gradient gel electrophoresis (DGGE), and restriction fragment length polymorphism (RFLP) analysis. The drained peatland showed low potential methane production and methanogen diversity in both control and ash-fertilized plots. Samples from both upper and deeper layers of peat were dominated by three groups of sequences related to Rice cluster-I hydrogenotroph methanogens. Even though pH was marginally greater in the ash-treated site, the occurrence of those sequences was not affected by ash fertilization. Interestingly, a less common group of se-quences, related to the Fen cluster, were found only in the fertilized plots. The study confirmed the depth related change of methanogen populations in peatland. Text Northern Finland Unknown |
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English |
description |
Forestry practises such has drainage have been shown to decrease emissions of the greenhouse gas methane (CH4) from peatlands. The aim of the study was to examine the methanogen populations in a drained bog in northern Finland, and to assess the possible effect of ash fertiliza-tion on potential methane production and methanogen communities. Peat samples were collected from control and ash fertilized (15,000 kg/ha) plots 5 years after ash application, and potential CH4 production was mea-sured. The methanogen community structure was stud-ied by DNA isolation, PCR amplification of the methyl coenzyme-M reductase (mcr) gene, denaturing gradient gel electrophoresis (DGGE), and restriction fragment length polymorphism (RFLP) analysis. The drained peatland showed low potential methane production and methanogen diversity in both control and ash-fertilized plots. Samples from both upper and deeper layers of peat were dominated by three groups of sequences related to Rice cluster-I hydrogenotroph methanogens. Even though pH was marginally greater in the ash-treated site, the occurrence of those sequences was not affected by ash fertilization. Interestingly, a less common group of se-quences, related to the Fen cluster, were found only in the fertilized plots. The study confirmed the depth related change of methanogen populations in peatland. |
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The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
P. E. Gal H. Juottonen H. Fritze |
spellingShingle |
P. E. Gal H. Juottonen H. Fritze Methanogen Communities in a Drained Bog: Effect of Ash Fertilization |
author_facet |
P. E. Gal H. Juottonen H. Fritze |
author_sort |
P. E. Gal |
title |
Methanogen Communities in a Drained Bog: Effect of Ash Fertilization |
title_short |
Methanogen Communities in a Drained Bog: Effect of Ash Fertilization |
title_full |
Methanogen Communities in a Drained Bog: Effect of Ash Fertilization |
title_fullStr |
Methanogen Communities in a Drained Bog: Effect of Ash Fertilization |
title_full_unstemmed |
Methanogen Communities in a Drained Bog: Effect of Ash Fertilization |
title_sort |
methanogen communities in a drained bog: effect of ash fertilization |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.464.6823 http://132.203.57.253/warwickvincent/PDFfiles/pierreMicrobialecol2005.pdf |
genre |
Northern Finland |
genre_facet |
Northern Finland |
op_source |
http://132.203.57.253/warwickvincent/PDFfiles/pierreMicrobialecol2005.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.464.6823 http://132.203.57.253/warwickvincent/PDFfiles/pierreMicrobialecol2005.pdf |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766144465126293504 |