Molecularanalysis of bacterial diversity in kerosene-based drilling £uid from the deep ice borehole at Vostok, East Antarctica
Decontamination of ice cores is a critical issue in phylogenetic studies of glacial ice and subglacial lakes. At the Vostok drill site, a total of 3650 m of ice core have now been obtained from the East Antarctic ice sheet. The ice core surface is coated with a hard-to-remove film of impure drilling...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.431.6681 2023-05-15T13:55:38+02:00 Molecularanalysis of bacterial diversity in kerosene-based drilling £uid from the deep ice borehole at Vostok, East Antarctica Irina A. Alekhina Dominique Marie Jean Robert Petit Valery V. Lukin Vladimir M. Zubkov Sergey A. Bulat Correspondence Irina A. Alekhina The Pennsylvania State University CiteSeerX Archives 2006 http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.431.6681 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.431.6681 Metadata may be used without restrictions as long as the oai identifier remains attached to it. Lake Vostok contamination Sphingomonas Antarctica extremophiles text 2006 ftciteseerx 2016-01-08T04:42:27Z Decontamination of ice cores is a critical issue in phylogenetic studies of glacial ice and subglacial lakes. At the Vostok drill site, a total of 3650 m of ice core have now been obtained from the East Antarctic ice sheet. The ice core surface is coated with a hard-to-remove film of impure drilling fluid comprising a mixture of aliphatic and aromatic hydrocarbons and foranes. In the present study we used 16S rRNA gene sequencing to analyze the bacterial content of the Vostok drilling fluid sampled from four depths in the borehole. Six phylotypes were identified in three of four samples studied. The two dominant phylotypes recovered from the deepest (3400 and 3600 m) and comparatively warm ( 10 1C and 6 1C, respectively) borehole horizons were from within the genus Sphingomonas, a well-known degrader of polyaromatic hydrocarbons. The remaining phylotypes encountered in all samples proved to be human- or soil-associated bacteria and were presumed to be drilling fluid contaminants of rare occurrence. The results obtained indicate the persistence of bacteria in extremely cold, hydrocarbon-rich environments. They show the potential for contamination of ice and subglacial water samples during lake exploration, and the need to develop a microbiological database of drilling fluid findings. Text Antarc* Antarctic Antarctica East Antarctica ice core Ice Sheet Unknown Antarctic East Antarctica East Antarctic Ice Sheet Lake Vostok ENVELOPE(106.000,106.000,-77.500,-77.500) |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftciteseerx |
language |
English |
topic |
Lake Vostok contamination Sphingomonas Antarctica extremophiles |
spellingShingle |
Lake Vostok contamination Sphingomonas Antarctica extremophiles Irina A. Alekhina Dominique Marie Jean Robert Petit Valery V. Lukin Vladimir M. Zubkov Sergey A. Bulat Correspondence Irina A. Alekhina Molecularanalysis of bacterial diversity in kerosene-based drilling £uid from the deep ice borehole at Vostok, East Antarctica |
topic_facet |
Lake Vostok contamination Sphingomonas Antarctica extremophiles |
description |
Decontamination of ice cores is a critical issue in phylogenetic studies of glacial ice and subglacial lakes. At the Vostok drill site, a total of 3650 m of ice core have now been obtained from the East Antarctic ice sheet. The ice core surface is coated with a hard-to-remove film of impure drilling fluid comprising a mixture of aliphatic and aromatic hydrocarbons and foranes. In the present study we used 16S rRNA gene sequencing to analyze the bacterial content of the Vostok drilling fluid sampled from four depths in the borehole. Six phylotypes were identified in three of four samples studied. The two dominant phylotypes recovered from the deepest (3400 and 3600 m) and comparatively warm ( 10 1C and 6 1C, respectively) borehole horizons were from within the genus Sphingomonas, a well-known degrader of polyaromatic hydrocarbons. The remaining phylotypes encountered in all samples proved to be human- or soil-associated bacteria and were presumed to be drilling fluid contaminants of rare occurrence. The results obtained indicate the persistence of bacteria in extremely cold, hydrocarbon-rich environments. They show the potential for contamination of ice and subglacial water samples during lake exploration, and the need to develop a microbiological database of drilling fluid findings. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Irina A. Alekhina Dominique Marie Jean Robert Petit Valery V. Lukin Vladimir M. Zubkov Sergey A. Bulat Correspondence Irina A. Alekhina |
author_facet |
Irina A. Alekhina Dominique Marie Jean Robert Petit Valery V. Lukin Vladimir M. Zubkov Sergey A. Bulat Correspondence Irina A. Alekhina |
author_sort |
Irina A. Alekhina |
title |
Molecularanalysis of bacterial diversity in kerosene-based drilling £uid from the deep ice borehole at Vostok, East Antarctica |
title_short |
Molecularanalysis of bacterial diversity in kerosene-based drilling £uid from the deep ice borehole at Vostok, East Antarctica |
title_full |
Molecularanalysis of bacterial diversity in kerosene-based drilling £uid from the deep ice borehole at Vostok, East Antarctica |
title_fullStr |
Molecularanalysis of bacterial diversity in kerosene-based drilling £uid from the deep ice borehole at Vostok, East Antarctica |
title_full_unstemmed |
Molecularanalysis of bacterial diversity in kerosene-based drilling £uid from the deep ice borehole at Vostok, East Antarctica |
title_sort |
molecularanalysis of bacterial diversity in kerosene-based drilling £uid from the deep ice borehole at vostok, east antarctica |
publishDate |
2006 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.431.6681 |
long_lat |
ENVELOPE(106.000,106.000,-77.500,-77.500) |
geographic |
Antarctic East Antarctica East Antarctic Ice Sheet Lake Vostok |
geographic_facet |
Antarctic East Antarctica East Antarctic Ice Sheet Lake Vostok |
genre |
Antarc* Antarctic Antarctica East Antarctica ice core Ice Sheet |
genre_facet |
Antarc* Antarctic Antarctica East Antarctica ice core Ice Sheet |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.431.6681 |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766262408273199104 |