2006. Archaeal uptake of enantiomeric amino acids in meso- and bathypelagic waters of the North
We determined the contribution of the three major prokaryotic groups (Bacteria, Crenarchaeota, and Euryarchaeota) on the uptake of D- and L-aspartic acid (Asp) in the major water masses of the North Atlantic (from 100to 4,000-m depth) with the use of microautoradiography combined with catalyzed repo...
Main Authors: | , , , |
---|---|
Other Authors: | |
Format: | Text |
Language: | English |
Subjects: | |
Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.419.4352 http://www.aslo.org/lo/toc/vol_51/issue_1/0060.pdf |
id |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.419.4352 |
---|---|
record_format |
openpolar |
spelling |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.419.4352 2023-05-15T17:13:54+02:00 2006. Archaeal uptake of enantiomeric amino acids in meso- and bathypelagic waters of the North Eva Teira Hendrik Van Aken Cornelis Veth Gerhard J. Herndl The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.419.4352 http://www.aslo.org/lo/toc/vol_51/issue_1/0060.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.419.4352 http://www.aslo.org/lo/toc/vol_51/issue_1/0060.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.aslo.org/lo/toc/vol_51/issue_1/0060.pdf text ftciteseerx 2016-01-08T03:52:40Z We determined the contribution of the three major prokaryotic groups (Bacteria, Crenarchaeota, and Euryarchaeota) on the uptake of D- and L-aspartic acid (Asp) in the major water masses of the North Atlantic (from 100to 4,000-m depth) with the use of microautoradiography combined with catalyzed reporter deposition fluorescence in situ hybridization (MICRO-CARD-FISH). The percentage of prokaryotic cells that assimilated D- and L-Asp ranged from �5 % to 25%. In the meso- and bathypelagic waters of the North Atlantic, Archaea are more abundant (42 % � 2 % of 4�,6�-diamino-2-phenylindole [DAPI]–stained cells) than Bacteria (30 % � 1 % of DAPI-stained cells), and more archaeal than bacterial cells are actively incorporating D-Asp (62 % � 2 % vs. 38 % � 2 % of total D-Asp active cells). In contrast, Bacteria and Archaea almost equally contribute to L-Asp use in the deep waters of the North Atlantic (47 % � 2 % vs. 53 % � 2 % of total L-Asp active cells). The increase in the D-Asp: L-Asp uptake ratio in the prokaryotic community with depth appears to be driven by the efficient uptake of D-Asp by, especially, the Crenarchaeota in the deep waters. Because Archaea, and particularly Crenarchaeota, commonly dominate the prokaryotic communities in the ocean’s interior, we suggest that they represent a previously unrecognized sink of D–amino acids in the deep ocean. The formation of the North Atlantic Deep Water (NADW) is the major driving force of the oceanic conveyor belt system that, in turn, influences the global climate (Broecker 1997). The turnover time of this oceanic conveyor belt system is about 2,000 yr, whereas that of the dissolved organic carbon (DOC) in the oceanic deep water is about 6,000– Text NADW North Atlantic Deep Water North Atlantic Unknown |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftciteseerx |
language |
English |
description |
We determined the contribution of the three major prokaryotic groups (Bacteria, Crenarchaeota, and Euryarchaeota) on the uptake of D- and L-aspartic acid (Asp) in the major water masses of the North Atlantic (from 100to 4,000-m depth) with the use of microautoradiography combined with catalyzed reporter deposition fluorescence in situ hybridization (MICRO-CARD-FISH). The percentage of prokaryotic cells that assimilated D- and L-Asp ranged from �5 % to 25%. In the meso- and bathypelagic waters of the North Atlantic, Archaea are more abundant (42 % � 2 % of 4�,6�-diamino-2-phenylindole [DAPI]–stained cells) than Bacteria (30 % � 1 % of DAPI-stained cells), and more archaeal than bacterial cells are actively incorporating D-Asp (62 % � 2 % vs. 38 % � 2 % of total D-Asp active cells). In contrast, Bacteria and Archaea almost equally contribute to L-Asp use in the deep waters of the North Atlantic (47 % � 2 % vs. 53 % � 2 % of total L-Asp active cells). The increase in the D-Asp: L-Asp uptake ratio in the prokaryotic community with depth appears to be driven by the efficient uptake of D-Asp by, especially, the Crenarchaeota in the deep waters. Because Archaea, and particularly Crenarchaeota, commonly dominate the prokaryotic communities in the ocean’s interior, we suggest that they represent a previously unrecognized sink of D–amino acids in the deep ocean. The formation of the North Atlantic Deep Water (NADW) is the major driving force of the oceanic conveyor belt system that, in turn, influences the global climate (Broecker 1997). The turnover time of this oceanic conveyor belt system is about 2,000 yr, whereas that of the dissolved organic carbon (DOC) in the oceanic deep water is about 6,000– |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Eva Teira Hendrik Van Aken Cornelis Veth Gerhard J. Herndl |
spellingShingle |
Eva Teira Hendrik Van Aken Cornelis Veth Gerhard J. Herndl 2006. Archaeal uptake of enantiomeric amino acids in meso- and bathypelagic waters of the North |
author_facet |
Eva Teira Hendrik Van Aken Cornelis Veth Gerhard J. Herndl |
author_sort |
Eva Teira |
title |
2006. Archaeal uptake of enantiomeric amino acids in meso- and bathypelagic waters of the North |
title_short |
2006. Archaeal uptake of enantiomeric amino acids in meso- and bathypelagic waters of the North |
title_full |
2006. Archaeal uptake of enantiomeric amino acids in meso- and bathypelagic waters of the North |
title_fullStr |
2006. Archaeal uptake of enantiomeric amino acids in meso- and bathypelagic waters of the North |
title_full_unstemmed |
2006. Archaeal uptake of enantiomeric amino acids in meso- and bathypelagic waters of the North |
title_sort |
2006. archaeal uptake of enantiomeric amino acids in meso- and bathypelagic waters of the north |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.419.4352 http://www.aslo.org/lo/toc/vol_51/issue_1/0060.pdf |
genre |
NADW North Atlantic Deep Water North Atlantic |
genre_facet |
NADW North Atlantic Deep Water North Atlantic |
op_source |
http://www.aslo.org/lo/toc/vol_51/issue_1/0060.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.419.4352 http://www.aslo.org/lo/toc/vol_51/issue_1/0060.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766071098353385472 |