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The kinetics of alcoholysis of methylpropionate and n-propanol catalyzed by Candida antarctica lipase B supported onto silanized Chromosorb P was studied in a continuous solid/gas reactor. In this system the solid phase is composed of a packed enzymatic sample and is percolated by nitrogen as carrie...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.372.7140 2023-05-15T13:38:20+02:00 KEY WORDS Marie-pierre Bousquet-dubouch Marianne Graber Nadine Sousa Sylvain Lamare Marie-dominique Legoy Lipase B From C Ping Pong Bi Bi The Pennsylvania State University CiteSeerX Archives 2008 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.372.7140 http://hal.archives-ouvertes.fr/docs/00/32/96/53/PDF/Publi1.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.372.7140 http://hal.archives-ouvertes.fr/docs/00/32/96/53/PDF/Publi1.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://hal.archives-ouvertes.fr/docs/00/32/96/53/PDF/Publi1.pdf text 2008 ftciteseerx 2016-01-08T01:23:12Z The kinetics of alcoholysis of methylpropionate and n-propanol catalyzed by Candida antarctica lipase B supported onto silanized Chromosorb P was studied in a continuous solid/gas reactor. In this system the solid phase is composed of a packed enzymatic sample and is percolated by nitrogen as carrier gas, which simultaneously carries substrates to the enzyme while removing reaction products. In this reactor the thermodynamic activity of substrates and effectors can be perfectly adjusted allowing kinetics studies to be performed under different operating conditions. The kinetics obtained for alcoholysis were suggested to hal-00329653, version 1- 13 Oct 2008 fit a Ping Pong Bi Bi mechanism with dead-end inhibition by the alcohol. The values of all apparent kinetic parameters were calculated and the apparent dissociation constant of enzyme for gaseous ester was found very low compared with the one obtained for liquid ester in organic medium, certainly due to the more efficient diffusion in the gaseous phase. The effect of water thermodynamic activity was also investigated. Water was found to act as a competitive inhibitor, with a higher inhibition constant than n-propanol. Thus alcoholysis of gaseous methylpropionate and n-propanol catalyzed by of Candida antarctica lipase B was found to obey the same kinetics mechanism than in other non conventional media such as organic liquid media and supercritical carbon dioxide, but with much higher affinity for the substrates. 2 Text Antarc* Antarctica Unknown |
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The kinetics of alcoholysis of methylpropionate and n-propanol catalyzed by Candida antarctica lipase B supported onto silanized Chromosorb P was studied in a continuous solid/gas reactor. In this system the solid phase is composed of a packed enzymatic sample and is percolated by nitrogen as carrier gas, which simultaneously carries substrates to the enzyme while removing reaction products. In this reactor the thermodynamic activity of substrates and effectors can be perfectly adjusted allowing kinetics studies to be performed under different operating conditions. The kinetics obtained for alcoholysis were suggested to hal-00329653, version 1- 13 Oct 2008 fit a Ping Pong Bi Bi mechanism with dead-end inhibition by the alcohol. The values of all apparent kinetic parameters were calculated and the apparent dissociation constant of enzyme for gaseous ester was found very low compared with the one obtained for liquid ester in organic medium, certainly due to the more efficient diffusion in the gaseous phase. The effect of water thermodynamic activity was also investigated. Water was found to act as a competitive inhibitor, with a higher inhibition constant than n-propanol. Thus alcoholysis of gaseous methylpropionate and n-propanol catalyzed by of Candida antarctica lipase B was found to obey the same kinetics mechanism than in other non conventional media such as organic liquid media and supercritical carbon dioxide, but with much higher affinity for the substrates. 2 |
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The Pennsylvania State University CiteSeerX Archives |
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Marie-pierre Bousquet-dubouch Marianne Graber Nadine Sousa Sylvain Lamare Marie-dominique Legoy Lipase B From C Ping Pong Bi Bi |
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Marie-pierre Bousquet-dubouch Marianne Graber Nadine Sousa Sylvain Lamare Marie-dominique Legoy Lipase B From C Ping Pong Bi Bi KEY WORDS |
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Marie-pierre Bousquet-dubouch Marianne Graber Nadine Sousa Sylvain Lamare Marie-dominique Legoy Lipase B From C Ping Pong Bi Bi |
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Marie-pierre Bousquet-dubouch |
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2008 |
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http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.372.7140 http://hal.archives-ouvertes.fr/docs/00/32/96/53/PDF/Publi1.pdf |
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Antarc* Antarctica |
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Antarc* Antarctica |
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http://hal.archives-ouvertes.fr/docs/00/32/96/53/PDF/Publi1.pdf |
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http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.372.7140 http://hal.archives-ouvertes.fr/docs/00/32/96/53/PDF/Publi1.pdf |
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1766104284636643328 |