medium; psychrotolerant; SDS PAGE. Correspondence
Fifty-six fungal isolates from Arctic soils were subjected to primary screening for their ability to solubilize insoluble inorganic phosphate. Nine of the isolates were further analysed quantitatively for phosphatase production using paranitrophenylphosphate as substrate. Amongst these, a cold-toler...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.361.1902 2023-05-15T15:04:43+02:00 medium; psychrotolerant; SDS PAGE. Correspondence Puja Gawas-sakhalkar Shiv Mohan Singh Simantini Naik Rasik Ravindra National Centre For The Pennsylvania State University CiteSeerX Archives http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.361.1902 http://www.polarresearch.net/index.php/polar/article/download/11105/pdf_1/ en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.361.1902 http://www.polarresearch.net/index.php/polar/article/download/11105/pdf_1/ Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.polarresearch.net/index.php/polar/article/download/11105/pdf_1/ enzyme para-nitrophenylphosphate Pikovskaya text ftciteseerx 2016-01-08T00:50:22Z Fifty-six fungal isolates from Arctic soils were subjected to primary screening for their ability to solubilize insoluble inorganic phosphate. Nine of the isolates were further analysed quantitatively for phosphatase production using paranitrophenylphosphate as substrate. Amongst these, a cold-tolerant fungus, Penicillium citrinum strain PG162 was found to be the best producer of intracellular acid phosphatase. Further characterization of the enzyme showed that it is most active in the temperature range of 40 608C and pH range of 4.2 4.8. The dried enzyme extract is stable at a temperature of up to 508C for at least 1 h. Its activity is affected by presence of metal ions. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) analysis suggests the molecular weight of the enzyme to be between 20 and 29 kDa. The present study is important with respect to our understanding of the kind of enzymatic reactions that take place in the polar microbes, and the extent to which their activity is sustained. Text Arctic Unknown Arctic |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftciteseerx |
language |
English |
topic |
enzyme para-nitrophenylphosphate Pikovskaya |
spellingShingle |
enzyme para-nitrophenylphosphate Pikovskaya Puja Gawas-sakhalkar Shiv Mohan Singh Simantini Naik Rasik Ravindra National Centre For medium; psychrotolerant; SDS PAGE. Correspondence |
topic_facet |
enzyme para-nitrophenylphosphate Pikovskaya |
description |
Fifty-six fungal isolates from Arctic soils were subjected to primary screening for their ability to solubilize insoluble inorganic phosphate. Nine of the isolates were further analysed quantitatively for phosphatase production using paranitrophenylphosphate as substrate. Amongst these, a cold-tolerant fungus, Penicillium citrinum strain PG162 was found to be the best producer of intracellular acid phosphatase. Further characterization of the enzyme showed that it is most active in the temperature range of 40 608C and pH range of 4.2 4.8. The dried enzyme extract is stable at a temperature of up to 508C for at least 1 h. Its activity is affected by presence of metal ions. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) analysis suggests the molecular weight of the enzyme to be between 20 and 29 kDa. The present study is important with respect to our understanding of the kind of enzymatic reactions that take place in the polar microbes, and the extent to which their activity is sustained. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Puja Gawas-sakhalkar Shiv Mohan Singh Simantini Naik Rasik Ravindra National Centre For |
author_facet |
Puja Gawas-sakhalkar Shiv Mohan Singh Simantini Naik Rasik Ravindra National Centre For |
author_sort |
Puja Gawas-sakhalkar |
title |
medium; psychrotolerant; SDS PAGE. Correspondence |
title_short |
medium; psychrotolerant; SDS PAGE. Correspondence |
title_full |
medium; psychrotolerant; SDS PAGE. Correspondence |
title_fullStr |
medium; psychrotolerant; SDS PAGE. Correspondence |
title_full_unstemmed |
medium; psychrotolerant; SDS PAGE. Correspondence |
title_sort |
medium; psychrotolerant; sds page. correspondence |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.361.1902 http://www.polarresearch.net/index.php/polar/article/download/11105/pdf_1/ |
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Arctic |
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Arctic |
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Arctic |
genre_facet |
Arctic |
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http://www.polarresearch.net/index.php/polar/article/download/11105/pdf_1/ |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.361.1902 http://www.polarresearch.net/index.php/polar/article/download/11105/pdf_1/ |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766336443694710784 |