Materials and methods Sampling

• Main Authors: Er V. Tkalin, T. S. Lishavskaya, L. T. Kovekovdova, M. V. Simakov, N. N. Bogdanova, T. L. Primak, E. A. Slin’ko, Bottom Sediments
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: Van Veen; Crassostrea gigas
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.359.8751; http://www.pices.int/publications/scientific_reports/Report16/ContaminantConcentration.pdf

Bottom sediment samples were collected by Van Veen grab from seven stations (Section I, Fig. 1.2). Three replicate samples were taken at each station. The surface layer of sediments was collected by plastic spoon in pre-cleaned Ziploc plastic bags. Samples were frozen after collection, then freeze-dried in the shore laboratory and transported to Russia for further analysis. Mussels Mussels (about 30 at each site) were collected from rocks and concrete piles during low tide at seven stations (Section I, Fig. 1.3). At all stations, mussels Mytilus trossolus were found. At station I-6, oysters Crassostrea gigas were also found. In the shore laboratory, soft tissues were removed, weighed, placed in pre-cleaned plastic containers and stored frozen. Then samples were freezedried and transported to Russia for further analysis. Fish Fish were collected by bottom trawl at 5 stations (Section I, Fig. 1.4). Fish muscle samples were taken from 5 individuals (English sole) at each trawling station and kept in pre-cleaned plastic bags on ice aboard the research vessel and then frozen in the shore laboratory. After freezedrying, samples were transported to Russia for further analysis. Analysis In Vladivostok (Russia), samples of bottom sediments, mussel and fish tissues were homogenized and distributed for analysis in three laboratories: