Research Diastereomers of the Brominated Flame Retardant 1,2-Dibromo-4-(1,2 dibromoethyl)cyclohexane Induce Androgen Receptor Activation in the HepG2 Hepatocellular Carcinoma Cell Line and the LNCaP Prostate Cancer Cell Line
Bac k g r o u n d: Reported incidences of prostate cancer and masculinization of animals indicate a release of compounds with androgenic properties into the environment. Large numbers of environmental pollutants have been screened to identify such compounds; however, not until recently was 1,2-dibro...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.356.9968 2023-05-15T15:41:56+02:00 Research Diastereomers of the Brominated Flame Retardant 1,2-Dibromo-4-(1,2 dibromoethyl)cyclohexane Induce Androgen Receptor Activation in the HepG2 Hepatocellular Carcinoma Cell Line and the LNCaP Prostate Cancer Cell Line Hazem Khalaf Anders Larsson Håkan Berg Robert Mccrindle Gilles Arsenault Per-erik Olsson The Pennsylvania State University CiteSeerX Archives application/zip http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.356.9968 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.356.9968 Metadata may be used without restrictions as long as the oai identifier remains attached to it. ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/4e/51/Environ_Health_Perspect_2009_Dec_3_117(12)_1853-1859.tar.gz text ftciteseerx 2016-01-08T00:37:10Z Bac k g r o u n d: Reported incidences of prostate cancer and masculinization of animals indicate a release of compounds with androgenic properties into the environment. Large numbers of environmental pollutants have been screened to identify such compounds; however, not until recently was 1,2-dibromo-4-(1,2-dibromoethyl)cyclohexane (TBECH) identified as the first potent activator of the human androgen receptor (hAR). TBECH has been found in beluga whales and bird eggs and has also been found to be maternally transferred in zebrafish. Objectives: In the present study we investigated interaction energies between TBECH diastereomers (α, β, γ, and δ) and the hAR, and their ability to activate the receptor and induce prostatespecific antigen (PSA) expression in vitro. Me t h o d s: We performed computational modeling to determine interaction energies between the ligand and the AR ligand-binding site, and measured in vitro competitive binding assays for AR by polarization fluorometry analysis. We used enzyme-linked immunosorbent assays to determine PSA activity in LNCaP and HepG2 cells. Re s u l t s: We found the γ and δ diastereomers to be more potent activators of hAR than the α and β diastereomers, which was confirmed in receptor binding studies. All TBECH diastereomers induced PSA expression in LNCaP cells even though the AR present in these cells is mutated (T877A). Modeling studies of LNCaP AR revealed that TBECH diastereomers bound to the receptor with a closer distance to the key amino acids in the ligand-binding domain, indicating stronger binding to the mutated receptor. Co n c l u s i o n s: The present study demonstrates the ability of TBECH to activate the hAR, indicating that it is a potential endocrine disruptor. Key w o r d s: androgen, brominated flame retardant, endocrine disruptor. Environ Health Perspect 117:1853–1859 (2009). doi:10.1289/ehp.0901065 available via Text Beluga Beluga* Unknown |
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Bac k g r o u n d: Reported incidences of prostate cancer and masculinization of animals indicate a release of compounds with androgenic properties into the environment. Large numbers of environmental pollutants have been screened to identify such compounds; however, not until recently was 1,2-dibromo-4-(1,2-dibromoethyl)cyclohexane (TBECH) identified as the first potent activator of the human androgen receptor (hAR). TBECH has been found in beluga whales and bird eggs and has also been found to be maternally transferred in zebrafish. Objectives: In the present study we investigated interaction energies between TBECH diastereomers (α, β, γ, and δ) and the hAR, and their ability to activate the receptor and induce prostatespecific antigen (PSA) expression in vitro. Me t h o d s: We performed computational modeling to determine interaction energies between the ligand and the AR ligand-binding site, and measured in vitro competitive binding assays for AR by polarization fluorometry analysis. We used enzyme-linked immunosorbent assays to determine PSA activity in LNCaP and HepG2 cells. Re s u l t s: We found the γ and δ diastereomers to be more potent activators of hAR than the α and β diastereomers, which was confirmed in receptor binding studies. All TBECH diastereomers induced PSA expression in LNCaP cells even though the AR present in these cells is mutated (T877A). Modeling studies of LNCaP AR revealed that TBECH diastereomers bound to the receptor with a closer distance to the key amino acids in the ligand-binding domain, indicating stronger binding to the mutated receptor. Co n c l u s i o n s: The present study demonstrates the ability of TBECH to activate the hAR, indicating that it is a potential endocrine disruptor. Key w o r d s: androgen, brominated flame retardant, endocrine disruptor. Environ Health Perspect 117:1853–1859 (2009). doi:10.1289/ehp.0901065 available via |
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The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Hazem Khalaf Anders Larsson Håkan Berg Robert Mccrindle Gilles Arsenault Per-erik Olsson |
spellingShingle |
Hazem Khalaf Anders Larsson Håkan Berg Robert Mccrindle Gilles Arsenault Per-erik Olsson Research Diastereomers of the Brominated Flame Retardant 1,2-Dibromo-4-(1,2 dibromoethyl)cyclohexane Induce Androgen Receptor Activation in the HepG2 Hepatocellular Carcinoma Cell Line and the LNCaP Prostate Cancer Cell Line |
author_facet |
Hazem Khalaf Anders Larsson Håkan Berg Robert Mccrindle Gilles Arsenault Per-erik Olsson |
author_sort |
Hazem Khalaf |
title |
Research Diastereomers of the Brominated Flame Retardant 1,2-Dibromo-4-(1,2 dibromoethyl)cyclohexane Induce Androgen Receptor Activation in the HepG2 Hepatocellular Carcinoma Cell Line and the LNCaP Prostate Cancer Cell Line |
title_short |
Research Diastereomers of the Brominated Flame Retardant 1,2-Dibromo-4-(1,2 dibromoethyl)cyclohexane Induce Androgen Receptor Activation in the HepG2 Hepatocellular Carcinoma Cell Line and the LNCaP Prostate Cancer Cell Line |
title_full |
Research Diastereomers of the Brominated Flame Retardant 1,2-Dibromo-4-(1,2 dibromoethyl)cyclohexane Induce Androgen Receptor Activation in the HepG2 Hepatocellular Carcinoma Cell Line and the LNCaP Prostate Cancer Cell Line |
title_fullStr |
Research Diastereomers of the Brominated Flame Retardant 1,2-Dibromo-4-(1,2 dibromoethyl)cyclohexane Induce Androgen Receptor Activation in the HepG2 Hepatocellular Carcinoma Cell Line and the LNCaP Prostate Cancer Cell Line |
title_full_unstemmed |
Research Diastereomers of the Brominated Flame Retardant 1,2-Dibromo-4-(1,2 dibromoethyl)cyclohexane Induce Androgen Receptor Activation in the HepG2 Hepatocellular Carcinoma Cell Line and the LNCaP Prostate Cancer Cell Line |
title_sort |
research diastereomers of the brominated flame retardant 1,2-dibromo-4-(1,2 dibromoethyl)cyclohexane induce androgen receptor activation in the hepg2 hepatocellular carcinoma cell line and the lncap prostate cancer cell line |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.356.9968 |
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Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/4e/51/Environ_Health_Perspect_2009_Dec_3_117(12)_1853-1859.tar.gz |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.356.9968 |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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