MOLECULAR PHYLOGENETICS AND DIAGNOSIS OF ANISAKIS, PSEUDOTERRANOVA, AND CONTRACAECUM FROM NORTHERN PACIFIC MARINE MAMMALS
ABSTRACT: Individual specimens of Anisakis, Pseudoterranova, and Contracaecum collected from marine mammals inhabiting northern Pacific waters were used for comparative diagnostic and molecular phylogenetic analyses. Forty-eight new sequences were obtained for this study of 14 Anisakis taxa, 8 Pseud...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.330.6458 2023-05-15T16:05:22+02:00 MOLECULAR PHYLOGENETICS AND DIAGNOSIS OF ANISAKIS, PSEUDOTERRANOVA, AND CONTRACAECUM FROM NORTHERN PACIFIC MARINE MAMMALS Steven A. Nadler Murray D. Dailey Lia Paggi Ra Siu Judy A. Sakanari The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.330.6458 http://nematol.unh.edu/paper/NadlerETAL_JP05.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.330.6458 http://nematol.unh.edu/paper/NadlerETAL_JP05.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://nematol.unh.edu/paper/NadlerETAL_JP05.pdf text ftciteseerx 2016-09-04T00:40:42Z ABSTRACT: Individual specimens of Anisakis, Pseudoterranova, and Contracaecum collected from marine mammals inhabiting northern Pacific waters were used for comparative diagnostic and molecular phylogenetic analyses. Forty-eight new sequences were obtained for this study of 14 Anisakis taxa, 8 Pseudoterranova taxa, 4 Contracaecum taxa, and 4 outgroup species. Partial 28S (LSU) and complete internal transcribed spacer (ITS-1, 5.8S, ITS-2) ribosomal DNA was amplified by the polymerase chain reaction and sequenced. Sequences of ITS indicated that Pseudoterranova specimens from Zalophus californianus (California sea lion), Mirounga angustirostris (northern elephant seal), Phoca vitulina (harbor seal), Enhydra lutris (sea otter), and Eumetopias jubatus (Steller’s sea lion) exactly matched P. decipiens s. str., extending the host and geographic range of this species. Anisakis from northern Pacific marine mammals were most closely related to members of the A. simplex species complex. Comparison of Anisakis ITS sequences diagnosed the presence of A. simplex Cin2M. angustirostris hosts, which is a new host record. Anisakis specimens from Phocoena phocoena (harbor porpoise), Lissodelphis borealis (Pacific rightwhale porpoise), and E. jubatus included 3 ITS sequences that did not match any known species. Contracaecum adults obtained from Z. californianus were most closely related to C. ogmorhini s.l. and C. rudolphii, but ITS sequences of these Contracaecum specimens did not match C. ogmorhini s. str. or C. margolisi. These novel Anisakis and Contracaecum ITS sequences may represent previously uncharacterized species. Phylogenetic analysis of LSU sequences revealed strong support for the monophyly of Anisakinae, Contracaecum plus Phocascaris, Pseudoterranova, and Anisakis. Phylogenetic trees inferred from ITS sequences yielded robustly Text Elephant Seal harbor seal Phoca vitulina Phocoena phocoena Unknown Pacific |
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ftciteseerx |
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English |
description |
ABSTRACT: Individual specimens of Anisakis, Pseudoterranova, and Contracaecum collected from marine mammals inhabiting northern Pacific waters were used for comparative diagnostic and molecular phylogenetic analyses. Forty-eight new sequences were obtained for this study of 14 Anisakis taxa, 8 Pseudoterranova taxa, 4 Contracaecum taxa, and 4 outgroup species. Partial 28S (LSU) and complete internal transcribed spacer (ITS-1, 5.8S, ITS-2) ribosomal DNA was amplified by the polymerase chain reaction and sequenced. Sequences of ITS indicated that Pseudoterranova specimens from Zalophus californianus (California sea lion), Mirounga angustirostris (northern elephant seal), Phoca vitulina (harbor seal), Enhydra lutris (sea otter), and Eumetopias jubatus (Steller’s sea lion) exactly matched P. decipiens s. str., extending the host and geographic range of this species. Anisakis from northern Pacific marine mammals were most closely related to members of the A. simplex species complex. Comparison of Anisakis ITS sequences diagnosed the presence of A. simplex Cin2M. angustirostris hosts, which is a new host record. Anisakis specimens from Phocoena phocoena (harbor porpoise), Lissodelphis borealis (Pacific rightwhale porpoise), and E. jubatus included 3 ITS sequences that did not match any known species. Contracaecum adults obtained from Z. californianus were most closely related to C. ogmorhini s.l. and C. rudolphii, but ITS sequences of these Contracaecum specimens did not match C. ogmorhini s. str. or C. margolisi. These novel Anisakis and Contracaecum ITS sequences may represent previously uncharacterized species. Phylogenetic analysis of LSU sequences revealed strong support for the monophyly of Anisakinae, Contracaecum plus Phocascaris, Pseudoterranova, and Anisakis. Phylogenetic trees inferred from ITS sequences yielded robustly |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Steven A. Nadler Murray D. Dailey Lia Paggi Ra Siu Judy A. Sakanari |
spellingShingle |
Steven A. Nadler Murray D. Dailey Lia Paggi Ra Siu Judy A. Sakanari MOLECULAR PHYLOGENETICS AND DIAGNOSIS OF ANISAKIS, PSEUDOTERRANOVA, AND CONTRACAECUM FROM NORTHERN PACIFIC MARINE MAMMALS |
author_facet |
Steven A. Nadler Murray D. Dailey Lia Paggi Ra Siu Judy A. Sakanari |
author_sort |
Steven A. Nadler |
title |
MOLECULAR PHYLOGENETICS AND DIAGNOSIS OF ANISAKIS, PSEUDOTERRANOVA, AND CONTRACAECUM FROM NORTHERN PACIFIC MARINE MAMMALS |
title_short |
MOLECULAR PHYLOGENETICS AND DIAGNOSIS OF ANISAKIS, PSEUDOTERRANOVA, AND CONTRACAECUM FROM NORTHERN PACIFIC MARINE MAMMALS |
title_full |
MOLECULAR PHYLOGENETICS AND DIAGNOSIS OF ANISAKIS, PSEUDOTERRANOVA, AND CONTRACAECUM FROM NORTHERN PACIFIC MARINE MAMMALS |
title_fullStr |
MOLECULAR PHYLOGENETICS AND DIAGNOSIS OF ANISAKIS, PSEUDOTERRANOVA, AND CONTRACAECUM FROM NORTHERN PACIFIC MARINE MAMMALS |
title_full_unstemmed |
MOLECULAR PHYLOGENETICS AND DIAGNOSIS OF ANISAKIS, PSEUDOTERRANOVA, AND CONTRACAECUM FROM NORTHERN PACIFIC MARINE MAMMALS |
title_sort |
molecular phylogenetics and diagnosis of anisakis, pseudoterranova, and contracaecum from northern pacific marine mammals |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.330.6458 http://nematol.unh.edu/paper/NadlerETAL_JP05.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Elephant Seal harbor seal Phoca vitulina Phocoena phocoena |
genre_facet |
Elephant Seal harbor seal Phoca vitulina Phocoena phocoena |
op_source |
http://nematol.unh.edu/paper/NadlerETAL_JP05.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.330.6458 http://nematol.unh.edu/paper/NadlerETAL_JP05.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766401256018935808 |