WAP65 Characterisation of the Warm Acclimated Protein gene (wap65) in the Antarctic plunderfish (Harpagifer antarcticus)

Physiological adaptation to increased environmental temperatures has been studied experimentally in a number of fish species, with the up-regulation of several genes identified as being associated with the process, such as the warm-acclimated protein (wap-65). This article describes the cloning and...

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Bibliographic Details
Main Authors: Melody S Clark, Gavin Burns
Other Authors: The Pennsylvania State University CiteSeerX Archives
Format: Text
Language:English
Subjects:
Online Access:http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.329.8204
http://nora.nerc.ac.uk/3956/1/Wap_65.pdf
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Summary:Physiological adaptation to increased environmental temperatures has been studied experimentally in a number of fish species, with the up-regulation of several genes identified as being associated with the process, such as the warm-acclimated protein (wap-65). This article describes the cloning and characterisation of the wap65-2 gene from the Antarctic plunderfish (Harpagifer antarcticus). The transcriptional expression of this gene in response to elevated seawater temperatures over a time course series is presented. Initially there is strong down regulation of this gene to a maximum of 40 fold within 4 hours, followed by recovery to almost control levels within 48 hours, indicating that this gene does not play a role in the potential temperature adaptation of H. antarcticus. 2 WAP65