Summary
We have examined at the molecular level the CDR3 and adjacent regions in peripheral blood B lymphocytes ofnormal individuals. A total of 111 sequences (12-28 sequences from six individuals) were obtained after cloning of the polymerase chain reaction-amplified segments into plasmids or phage. The av...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.326.5868 2023-05-15T16:02:00+02:00 Summary Blood B Lymphocytes Masao Yamada Robert Wasserman Betty Anne Reichard Sara Shane Andrew J Caton Giovanni Rovera The Pennsylvania State University CiteSeerX Archives 1991 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.326.5868 http://jem.rupress.org/content/173/2/395.full.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.326.5868 http://jem.rupress.org/content/173/2/395.full.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://jem.rupress.org/content/173/2/395.full.pdf text 1991 ftciteseerx 2016-09-04T00:30:47Z We have examined at the molecular level the CDR3 and adjacent regions in peripheral blood B lymphocytes ofnormal individuals. A total of 111 sequences (12-28 sequences from six individuals) were obtained after cloning of the polymerase chain reaction-amplified segments into plasmids or phage. The average length of the VDJ joining was 109 nucleotides, with a range from 79 to 151. Approximately 75 % of the sequences were in frame when translated into amino acids. Among the JH segments, J H4 was found most frequently (in 52.5 % of the sequences), and JH 1 and JH 2 segments the least frequently (-I % of the clones). A polymorphic JH6 gene with a one-codnn deletion accompanied by a base change was present in two of six patients. Preferential breakpoints were found for JH 2, J.3, J 4, and Jx5, although the breakpoints of JH6 were distributed more heterogenously. In-90 % of the cases, significant homology of the D regions with published D sequences was found. Preferential usage of a particular coding frame was observed in in-frame sequences utilizing DA, D21/9, and DMl segments. However, in general, all coding frames of germline D genes were used to generate CDR3s. Eight sequences that have a DN1-like D sequence with two base changes at the same positions were identified, suggesting the likely existence of a new Text DML Unknown |
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We have examined at the molecular level the CDR3 and adjacent regions in peripheral blood B lymphocytes ofnormal individuals. A total of 111 sequences (12-28 sequences from six individuals) were obtained after cloning of the polymerase chain reaction-amplified segments into plasmids or phage. The average length of the VDJ joining was 109 nucleotides, with a range from 79 to 151. Approximately 75 % of the sequences were in frame when translated into amino acids. Among the JH segments, J H4 was found most frequently (in 52.5 % of the sequences), and JH 1 and JH 2 segments the least frequently (-I % of the clones). A polymorphic JH6 gene with a one-codnn deletion accompanied by a base change was present in two of six patients. Preferential breakpoints were found for JH 2, J.3, J 4, and Jx5, although the breakpoints of JH6 were distributed more heterogenously. In-90 % of the cases, significant homology of the D regions with published D sequences was found. Preferential usage of a particular coding frame was observed in in-frame sequences utilizing DA, D21/9, and DMl segments. However, in general, all coding frames of germline D genes were used to generate CDR3s. Eight sequences that have a DN1-like D sequence with two base changes at the same positions were identified, suggesting the likely existence of a new |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Blood B Lymphocytes Masao Yamada Robert Wasserman Betty Anne Reichard Sara Shane Andrew J Caton Giovanni Rovera |
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Blood B Lymphocytes Masao Yamada Robert Wasserman Betty Anne Reichard Sara Shane Andrew J Caton Giovanni Rovera Summary |
author_facet |
Blood B Lymphocytes Masao Yamada Robert Wasserman Betty Anne Reichard Sara Shane Andrew J Caton Giovanni Rovera |
author_sort |
Blood B Lymphocytes |
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publishDate |
1991 |
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http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.326.5868 http://jem.rupress.org/content/173/2/395.full.pdf |
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http://jem.rupress.org/content/173/2/395.full.pdf |
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http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.326.5868 http://jem.rupress.org/content/173/2/395.full.pdf |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766397653855240192 |