Branchial Expression Patterns of Claudin Isoforms in Atlantic Salmon During
In euryhaline teleosts, permeability changes in gill epithelia are essential during acclimation to changed salinity. This study examined expression patterns of branchial tight junction proteins called claudins, which are important determinants of ion selectivity and general permeability in epithelia...
Main Authors: | , , , , , , |
---|---|
Other Authors: | |
Format: | Text |
Language: | English |
Subjects: | |
Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.326.2622 http://ajpregu.physiology.org/content/early/2008/03/05/ajpregu.00915.2007.full.pdf |
id |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.326.2622 |
---|---|
record_format |
openpolar |
spelling |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.326.2622 2023-05-15T15:31:19+02:00 Branchial Expression Patterns of Claudin Isoforms in Atlantic Salmon During Seawater Acclimation Ck Tipsmark P Kiilerich To Nilsen Loe Ebbesson So Stefansson Ss Madsen The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.326.2622 http://ajpregu.physiology.org/content/early/2008/03/05/ajpregu.00915.2007.full.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.326.2622 http://ajpregu.physiology.org/content/early/2008/03/05/ajpregu.00915.2007.full.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://ajpregu.physiology.org/content/early/2008/03/05/ajpregu.00915.2007.full.pdf text ftciteseerx 2016-09-04T00:30:09Z In euryhaline teleosts, permeability changes in gill epithelia are essential during acclimation to changed salinity. This study examined expression patterns of branchial tight junction proteins called claudins, which are important determinants of ion selectivity and general permeability in epithelia. We identified Atlantic salmon genes belonging to the claudin family by screening expressed sequence tag libraries available at NCBI and classification was performed with aid of maximum likelihood and neighbour-joining analysis. In gill libraries, five isoforms (10e, 27a, 28a, 28b and 30) were present and QPCR analysis confirmed tissue-specific expression in gill when compared to kidney, intestine, heart, muscle, brain and liver. Expression patterns during acclimation of freshwater salmon to seawater (SW) and during the smoltification process were examined. Acclimation to SW reduced the expression of claudin 27a and claudin 30 but had no overall effect on claudin 28a and claudin 28b. In contrast, SW induced a 4-fold increase in expression of claudin 10e. In accord, a peak in branchial claudin 10e was observed during smoltification in May, coinciding with optimal SW tolerance. Smoltification induced no significant changes in expression of the other isoforms. This study demonstrates the expression Text Atlantic salmon Unknown |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftciteseerx |
language |
English |
description |
In euryhaline teleosts, permeability changes in gill epithelia are essential during acclimation to changed salinity. This study examined expression patterns of branchial tight junction proteins called claudins, which are important determinants of ion selectivity and general permeability in epithelia. We identified Atlantic salmon genes belonging to the claudin family by screening expressed sequence tag libraries available at NCBI and classification was performed with aid of maximum likelihood and neighbour-joining analysis. In gill libraries, five isoforms (10e, 27a, 28a, 28b and 30) were present and QPCR analysis confirmed tissue-specific expression in gill when compared to kidney, intestine, heart, muscle, brain and liver. Expression patterns during acclimation of freshwater salmon to seawater (SW) and during the smoltification process were examined. Acclimation to SW reduced the expression of claudin 27a and claudin 30 but had no overall effect on claudin 28a and claudin 28b. In contrast, SW induced a 4-fold increase in expression of claudin 10e. In accord, a peak in branchial claudin 10e was observed during smoltification in May, coinciding with optimal SW tolerance. Smoltification induced no significant changes in expression of the other isoforms. This study demonstrates the expression |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Seawater Acclimation Ck Tipsmark P Kiilerich To Nilsen Loe Ebbesson So Stefansson Ss Madsen |
spellingShingle |
Seawater Acclimation Ck Tipsmark P Kiilerich To Nilsen Loe Ebbesson So Stefansson Ss Madsen Branchial Expression Patterns of Claudin Isoforms in Atlantic Salmon During |
author_facet |
Seawater Acclimation Ck Tipsmark P Kiilerich To Nilsen Loe Ebbesson So Stefansson Ss Madsen |
author_sort |
Seawater Acclimation |
title |
Branchial Expression Patterns of Claudin Isoforms in Atlantic Salmon During |
title_short |
Branchial Expression Patterns of Claudin Isoforms in Atlantic Salmon During |
title_full |
Branchial Expression Patterns of Claudin Isoforms in Atlantic Salmon During |
title_fullStr |
Branchial Expression Patterns of Claudin Isoforms in Atlantic Salmon During |
title_full_unstemmed |
Branchial Expression Patterns of Claudin Isoforms in Atlantic Salmon During |
title_sort |
branchial expression patterns of claudin isoforms in atlantic salmon during |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.326.2622 http://ajpregu.physiology.org/content/early/2008/03/05/ajpregu.00915.2007.full.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
http://ajpregu.physiology.org/content/early/2008/03/05/ajpregu.00915.2007.full.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.326.2622 http://ajpregu.physiology.org/content/early/2008/03/05/ajpregu.00915.2007.full.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766361805558382592 |