Characterization of phocid herpesvirus-1 and-2 as putative alpha- and gammaherpesviruses of North American and European pinnipeds

• Main Authors: T. C. Harder, M. Harder, H. Vos, K. Kulonen, S. Kennedy-stoskopf, B. Liess, A. D. M. E. Osterhaus
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: American Harbour; Eia; Phoca vitulina
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.325.7663; http://vir.sgmjournals.org/content/77/1/27.full.pdf

To study the relationships between herpesviruses recently isolated from different pinniped species, antigenic and genetic analyses were performed. First, herpesviruses isolated from North American harbour seals (Phoca vitulina), a Californian sea lion (Zalophus californianus) and a European grey seal (Halichoerus grypus) were examined in an enzyme immunoassay (EIA) with a panel of monoclonal antibodies which had previously been shown to allow typing of herpesviruses from European harbour seals into two distinct virus types: phocid herpesvirus type-1 and type-2 (PhHV-1 and PhHV-2). The EIA data showed that all but one of the isolates from seals ranging in United States coastal waters were PhHV-2-1ike while the European grey seal herpesvirus was PhHV-l-like. Genetic characterization