Isolation of six putative P-type ATPase � subunit PCR fragments from the brain of the European eel Ž Anguilla anguilla . Annals

• Main Authors: C. P. Cutler, I. L. Sanders, G. Crambb, Ky Ts
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Published: 1997
• Subjects: Anguilla anguilla
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.325.1648; http://www.bio.georgiasouthern.edu/Bio-home/Cutler/Cutler Sanders Cramb P-type B subunits.pdf

Over the last decade substantial progress has been made in the identification of isoforms of P-type ATPase subunits. Of the P-type ATPases characterized, only the four cx subunit isoforms of Na,K-ATPasel or the two a subunit isoforms of the closely related enzyme H,K-ATPase2 are known to associate with p subunits. To date, studies investigating the existence of P-type ATPase p subunit isoforms have resulted in the identification of five p subunit isoforms (designated pl, p2, p3, H,K p, and pbl) in different specie^. ^ However, unlike the Na,K-ATPase or H,K-ATPase a subunit isoforms which share relatively high levels of amino acid homology (>60%), the amino acid sequences of p subunit isoforms share much lower levels of homology, sometimes as low as 30%. The low level of homology of p subunit isoforms makes the task of discovering new isoforms considerably more difficult. Here as part of a wider study investigating the role of ion transporters in various osmoregulatory tissues in euryhaline fish, experiments were designed to determine which P-type ATPase p subunit isoforms were expressed in the European eel (Anguilla anguilla). RESULTS AND DISCUSSION