Induction of Cetacean Cytochrome P4501A1 by b-Naphthoflavone Exposure of Skin Biopsy Slices
Marine mammals can accumulate environmental contaminants in their blubber at concentrations harmful to laboratory animals. Induction of the cytochrome P450 1A1 (CYP1A1) enzyme is widely used as a biomarker of exposure and molecular effects in animal species, yet the validity of this biomarker has no...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.324.6726 2023-05-15T17:59:23+02:00 Induction of Cetacean Cytochrome P4501A1 by b-Naphthoflavone Exposure of Skin Biopsy Slices The Pennsylvania State University CiteSeerX Archives 2003 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.324.6726 http://www.whoi.edu/science/seagrant/Publications/ocean/Godard.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.324.6726 http://www.whoi.edu/science/seagrant/Publications/ocean/Godard.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.whoi.edu/science/seagrant/Publications/ocean/Godard.pdf text 2003 ftciteseerx 2016-09-04T00:27:10Z Marine mammals can accumulate environmental contaminants in their blubber at concentrations harmful to laboratory animals. Induction of the cytochrome P450 1A1 (CYP1A1) enzyme is widely used as a biomarker of exposure and molecular effects in animal species, yet the validity of this biomarker has not been established in marine mammals. In vivo studies are generally precluded in these protected species, but skin biopsies (epidermis and dermis) can be collected in a minimally invasive way. We developed an in vitro assay using skin biopsy slices to examine CYP1A1 protein induction in marine mammals in response to chemical exposure. Skin biopsies from sperm whale (Physeter macrocephalus) were exposed for 24 h to b-naphthoflavone (BNF), a prototypical CYP1A1 inducer, and CYP1A1 induction was detected by immunohistochemical staining in endothelial cells, smooth muscle cells, and fibroblasts. Biopsy slices were exposed to a range of BNF concentrations (0.6–600 mM) Text Physeter macrocephalus Sperm whale Unknown |
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English |
description |
Marine mammals can accumulate environmental contaminants in their blubber at concentrations harmful to laboratory animals. Induction of the cytochrome P450 1A1 (CYP1A1) enzyme is widely used as a biomarker of exposure and molecular effects in animal species, yet the validity of this biomarker has not been established in marine mammals. In vivo studies are generally precluded in these protected species, but skin biopsies (epidermis and dermis) can be collected in a minimally invasive way. We developed an in vitro assay using skin biopsy slices to examine CYP1A1 protein induction in marine mammals in response to chemical exposure. Skin biopsies from sperm whale (Physeter macrocephalus) were exposed for 24 h to b-naphthoflavone (BNF), a prototypical CYP1A1 inducer, and CYP1A1 induction was detected by immunohistochemical staining in endothelial cells, smooth muscle cells, and fibroblasts. Biopsy slices were exposed to a range of BNF concentrations (0.6–600 mM) |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
title |
Induction of Cetacean Cytochrome P4501A1 by b-Naphthoflavone Exposure of Skin Biopsy Slices |
spellingShingle |
Induction of Cetacean Cytochrome P4501A1 by b-Naphthoflavone Exposure of Skin Biopsy Slices |
title_short |
Induction of Cetacean Cytochrome P4501A1 by b-Naphthoflavone Exposure of Skin Biopsy Slices |
title_full |
Induction of Cetacean Cytochrome P4501A1 by b-Naphthoflavone Exposure of Skin Biopsy Slices |
title_fullStr |
Induction of Cetacean Cytochrome P4501A1 by b-Naphthoflavone Exposure of Skin Biopsy Slices |
title_full_unstemmed |
Induction of Cetacean Cytochrome P4501A1 by b-Naphthoflavone Exposure of Skin Biopsy Slices |
title_sort |
induction of cetacean cytochrome p4501a1 by b-naphthoflavone exposure of skin biopsy slices |
publishDate |
2003 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.324.6726 http://www.whoi.edu/science/seagrant/Publications/ocean/Godard.pdf |
genre |
Physeter macrocephalus Sperm whale |
genre_facet |
Physeter macrocephalus Sperm whale |
op_source |
http://www.whoi.edu/science/seagrant/Publications/ocean/Godard.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.324.6726 http://www.whoi.edu/science/seagrant/Publications/ocean/Godard.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766168188933898240 |