HSP Antarctic molluscs Antarctic marine molluscs do have an HSP70 heat shock response 1
The success of any organism depends not only on niche adaptation, but also the ability to survive environmental perturbation from homeostasis, a situation generically described as stress. Although species-specific mechanisms to combat “stress ” have been described, the production of Heat Shock Prote...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.323.1224 2023-05-15T13:38:14+02:00 HSP Antarctic molluscs Antarctic marine molluscs do have an HSP70 heat shock response 1 Melody S. Clark Keiron P. P. Fraser Lloyd S. Peck The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.323.1224 http://nora.nerc.ac.uk/3954/1/Mollusc_HSP.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.323.1224 http://nora.nerc.ac.uk/3954/1/Mollusc_HSP.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://nora.nerc.ac.uk/3954/1/Mollusc_HSP.pdf Antarctic heat shock protein stress temperature climate change 1 HSP Antarctic molluscs Summary text ftciteseerx 2016-09-04T00:23:46Z The success of any organism depends not only on niche adaptation, but also the ability to survive environmental perturbation from homeostasis, a situation generically described as stress. Although species-specific mechanisms to combat “stress ” have been described, the production of Heat Shock Proteins (HSPs), such as HSP70 is universally described across all taxa. Members of the HSP70 gene family comprising the constitutive (HSC70) and inducible (HSP70) members, plus GRP78 (Glucose-regulated protein 78kDa), a related HSP70 family member were cloned using degenerate PCR from two evolutionary divergent Antarctic marine molluscs (Laternula elliptica and Nacella concinna), a bivalve and gastropod respectively. The expression of the HSP70 family members was surveyed via Q-PCR after an acute two-hour heat shock experiment. Both species demonstrated significant up-regulation of HSP70 gene expression in response to increased temperatures. However, the temperature level at which these responses were induced varied with the species (+6-8°C for L. elliptica and +8-10°C for N. concinna) compared to their natural environmental temparture). L. elliptica also showed tissuespecific expression of the genes under study. Previous work on Antarctic fish has shown that they lack the classical heat shock response: with the inducible form of HSP70 being permanently expressed with expression not further induced under higher temperature regimes. This study shows that this is not the case for other Antarctic animals, with the two molluscs showing an inducible heat shock response, at a level probably set during their temperate evolutionary past. 2 HSP Antarctic molluscs Text Antarc* Antarctic Unknown Antarctic Nacella ENVELOPE(-60.783,-60.783,-62.467,-62.467) |
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ftciteseerx |
language |
English |
topic |
Antarctic heat shock protein stress temperature climate change 1 HSP Antarctic molluscs Summary |
spellingShingle |
Antarctic heat shock protein stress temperature climate change 1 HSP Antarctic molluscs Summary Melody S. Clark Keiron P. P. Fraser Lloyd S. Peck HSP Antarctic molluscs Antarctic marine molluscs do have an HSP70 heat shock response 1 |
topic_facet |
Antarctic heat shock protein stress temperature climate change 1 HSP Antarctic molluscs Summary |
description |
The success of any organism depends not only on niche adaptation, but also the ability to survive environmental perturbation from homeostasis, a situation generically described as stress. Although species-specific mechanisms to combat “stress ” have been described, the production of Heat Shock Proteins (HSPs), such as HSP70 is universally described across all taxa. Members of the HSP70 gene family comprising the constitutive (HSC70) and inducible (HSP70) members, plus GRP78 (Glucose-regulated protein 78kDa), a related HSP70 family member were cloned using degenerate PCR from two evolutionary divergent Antarctic marine molluscs (Laternula elliptica and Nacella concinna), a bivalve and gastropod respectively. The expression of the HSP70 family members was surveyed via Q-PCR after an acute two-hour heat shock experiment. Both species demonstrated significant up-regulation of HSP70 gene expression in response to increased temperatures. However, the temperature level at which these responses were induced varied with the species (+6-8°C for L. elliptica and +8-10°C for N. concinna) compared to their natural environmental temparture). L. elliptica also showed tissuespecific expression of the genes under study. Previous work on Antarctic fish has shown that they lack the classical heat shock response: with the inducible form of HSP70 being permanently expressed with expression not further induced under higher temperature regimes. This study shows that this is not the case for other Antarctic animals, with the two molluscs showing an inducible heat shock response, at a level probably set during their temperate evolutionary past. 2 HSP Antarctic molluscs |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Melody S. Clark Keiron P. P. Fraser Lloyd S. Peck |
author_facet |
Melody S. Clark Keiron P. P. Fraser Lloyd S. Peck |
author_sort |
Melody S. Clark |
title |
HSP Antarctic molluscs Antarctic marine molluscs do have an HSP70 heat shock response 1 |
title_short |
HSP Antarctic molluscs Antarctic marine molluscs do have an HSP70 heat shock response 1 |
title_full |
HSP Antarctic molluscs Antarctic marine molluscs do have an HSP70 heat shock response 1 |
title_fullStr |
HSP Antarctic molluscs Antarctic marine molluscs do have an HSP70 heat shock response 1 |
title_full_unstemmed |
HSP Antarctic molluscs Antarctic marine molluscs do have an HSP70 heat shock response 1 |
title_sort |
hsp antarctic molluscs antarctic marine molluscs do have an hsp70 heat shock response 1 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.323.1224 http://nora.nerc.ac.uk/3954/1/Mollusc_HSP.pdf |
long_lat |
ENVELOPE(-60.783,-60.783,-62.467,-62.467) |
geographic |
Antarctic Nacella |
geographic_facet |
Antarctic Nacella |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
http://nora.nerc.ac.uk/3954/1/Mollusc_HSP.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.323.1224 http://nora.nerc.ac.uk/3954/1/Mollusc_HSP.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766103123760250880 |