from the Atlantic Cod, Gadus morhua
Abstract. Assembly of brain microtubule proteins isolated from the Atlantic cod, Gadus morhua, was found to be much less sensitive to colchicine than assembly of bovine brain microtubules, which was completely inhibited by low colchicine concentrations (10 #M). The degree of disassembly by colchicin...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.322.9811 2023-05-15T15:26:57+02:00 from the Atlantic Cod, Gadus morhua Stability Acetylated Cold-labile Brain Microtubules Martin Billger Margareta Wallin The Pennsylvania State University CiteSeerX Archives 1991 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.322.9811 http://jcb.rupress.org/content/113/2/331.full.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.322.9811 http://jcb.rupress.org/content/113/2/331.full.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://jcb.rupress.org/content/113/2/331.full.pdf text 1991 ftciteseerx 2016-09-04T00:23:40Z Abstract. Assembly of brain microtubule proteins isolated from the Atlantic cod, Gadus morhua, was found to be much less sensitive to colchicine than assembly of bovine brain microtubules, which was completely inhibited by low colchicine concentrations (10 #M). The degree of disassembly by colchicine was also less for cod microtubules. The lack of colchicine effect was not caused by a lower affinity of colchicine to cod tubulin, as colchicine bound to cod tubulin with a dissociation constant, Kd, and a binding ratio close to that of bovine tubulin. Cod brain tubulin was highly acetylated and mainly detyrosinated, as opposed to bovine tubulin. When cod tubulin, purified by means of phosphocellulose chromatography, was assembled by addition of DMSO Text atlantic cod Gadus morhua Unknown |
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ftciteseerx |
language |
English |
description |
Abstract. Assembly of brain microtubule proteins isolated from the Atlantic cod, Gadus morhua, was found to be much less sensitive to colchicine than assembly of bovine brain microtubules, which was completely inhibited by low colchicine concentrations (10 #M). The degree of disassembly by colchicine was also less for cod microtubules. The lack of colchicine effect was not caused by a lower affinity of colchicine to cod tubulin, as colchicine bound to cod tubulin with a dissociation constant, Kd, and a binding ratio close to that of bovine tubulin. Cod brain tubulin was highly acetylated and mainly detyrosinated, as opposed to bovine tubulin. When cod tubulin, purified by means of phosphocellulose chromatography, was assembled by addition of DMSO |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Stability Acetylated Cold-labile Brain Microtubules Martin Billger Margareta Wallin |
spellingShingle |
Stability Acetylated Cold-labile Brain Microtubules Martin Billger Margareta Wallin from the Atlantic Cod, Gadus morhua |
author_facet |
Stability Acetylated Cold-labile Brain Microtubules Martin Billger Margareta Wallin |
author_sort |
Stability Acetylated |
title |
from the Atlantic Cod, Gadus morhua |
title_short |
from the Atlantic Cod, Gadus morhua |
title_full |
from the Atlantic Cod, Gadus morhua |
title_fullStr |
from the Atlantic Cod, Gadus morhua |
title_full_unstemmed |
from the Atlantic Cod, Gadus morhua |
title_sort |
from the atlantic cod, gadus morhua |
publishDate |
1991 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.322.9811 http://jcb.rupress.org/content/113/2/331.full.pdf |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
http://jcb.rupress.org/content/113/2/331.full.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.322.9811 http://jcb.rupress.org/content/113/2/331.full.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766357413800181760 |