Levels in Atlantic Salmon After Waterborne Exposure to Nominal Environmental Concentrations of the Pharmaceutical Ethynylestradiol
In this study, the effects of two environmental endocrine disruptors, the synthetic pharmaceutical estrogen (ethynylestradiol, EE2) and antifoulant (tributyltin, TBT) representing two different modes of action on the endocrine system, were studied on brain steroidogenic pathway of juvenile Atlantic...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.321.8109 2023-05-15T15:30:44+02:00 Levels in Atlantic Salmon After Waterborne Exposure to Nominal Environmental Concentrations of the Pharmaceutical Ethynylestradiol Brain Cytochrome P Aromatase Gene Isoforms Antifoulant Tributyltin Angeliki Lyssimachou Bjørn Munro Jenssen Augustine Arukwe The Pennsylvania State University CiteSeerX Archives 2006 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.321.8109 http://toxsci.oxfordjournals.org/content/91/1/82.full.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.321.8109 http://toxsci.oxfordjournals.org/content/91/1/82.full.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://toxsci.oxfordjournals.org/content/91/1/82.full.pdf text 2006 ftciteseerx 2016-09-04T00:21:21Z In this study, the effects of two environmental endocrine disruptors, the synthetic pharmaceutical estrogen (ethynylestradiol, EE2) and antifoulant (tributyltin, TBT) representing two different modes of action on the endocrine system, were studied on brain steroidogenic pathway of juvenile Atlantic salmon (Salmo salar). Neurosteroidogenesis was studied using brain aromatase gene isoforms and enzyme activity, in parallel with typical xenoestrogen responses, such as brain estrogen receptor (ERa) and plasma vitellogenin (Vtg) levels. Fish were exposed to nominal waterborne EE2 (5 and 50 ng/l) and TBT (50 and 250 ng/l) concentrations dissolved in dimethyl sulfoxide (DMSO), singly and in combination. Gene expressions were quantified using real-time PCR with gene-specific primers, aromatase activity was analyzed using the tritiated water-release assay, and plasma Vtg was analyzed using competitive ELISA. Our data show that Text Atlantic salmon Salmo salar Unknown |
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language |
English |
description |
In this study, the effects of two environmental endocrine disruptors, the synthetic pharmaceutical estrogen (ethynylestradiol, EE2) and antifoulant (tributyltin, TBT) representing two different modes of action on the endocrine system, were studied on brain steroidogenic pathway of juvenile Atlantic salmon (Salmo salar). Neurosteroidogenesis was studied using brain aromatase gene isoforms and enzyme activity, in parallel with typical xenoestrogen responses, such as brain estrogen receptor (ERa) and plasma vitellogenin (Vtg) levels. Fish were exposed to nominal waterborne EE2 (5 and 50 ng/l) and TBT (50 and 250 ng/l) concentrations dissolved in dimethyl sulfoxide (DMSO), singly and in combination. Gene expressions were quantified using real-time PCR with gene-specific primers, aromatase activity was analyzed using the tritiated water-release assay, and plasma Vtg was analyzed using competitive ELISA. Our data show that |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Brain Cytochrome P Aromatase Gene Isoforms Antifoulant Tributyltin Angeliki Lyssimachou Bjørn Munro Jenssen Augustine Arukwe |
spellingShingle |
Brain Cytochrome P Aromatase Gene Isoforms Antifoulant Tributyltin Angeliki Lyssimachou Bjørn Munro Jenssen Augustine Arukwe Levels in Atlantic Salmon After Waterborne Exposure to Nominal Environmental Concentrations of the Pharmaceutical Ethynylestradiol |
author_facet |
Brain Cytochrome P Aromatase Gene Isoforms Antifoulant Tributyltin Angeliki Lyssimachou Bjørn Munro Jenssen Augustine Arukwe |
author_sort |
Brain Cytochrome |
title |
Levels in Atlantic Salmon After Waterborne Exposure to Nominal Environmental Concentrations of the Pharmaceutical Ethynylestradiol |
title_short |
Levels in Atlantic Salmon After Waterborne Exposure to Nominal Environmental Concentrations of the Pharmaceutical Ethynylestradiol |
title_full |
Levels in Atlantic Salmon After Waterborne Exposure to Nominal Environmental Concentrations of the Pharmaceutical Ethynylestradiol |
title_fullStr |
Levels in Atlantic Salmon After Waterborne Exposure to Nominal Environmental Concentrations of the Pharmaceutical Ethynylestradiol |
title_full_unstemmed |
Levels in Atlantic Salmon After Waterborne Exposure to Nominal Environmental Concentrations of the Pharmaceutical Ethynylestradiol |
title_sort |
levels in atlantic salmon after waterborne exposure to nominal environmental concentrations of the pharmaceutical ethynylestradiol |
publishDate |
2006 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.321.8109 http://toxsci.oxfordjournals.org/content/91/1/82.full.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
http://toxsci.oxfordjournals.org/content/91/1/82.full.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.321.8109 http://toxsci.oxfordjournals.org/content/91/1/82.full.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766361188129570816 |