Provincia de Tierra del Fuego (Argentina)
Summary. Peroxidases play a major role in several physiological processes. Specifically, in vitro activity of this enzyme was used as a marker to identify different rooting phases of Nothofagus. The study of peroxidase profile patterns during rhizogenesis in this genus could give additional informat...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.318.960 2023-05-15T13:59:51+02:00 Provincia de Tierra del Fuego (Argentina) Laboratorio De Fisiologia Vegetal G. Martinez Pastur The Pennsylvania State University CiteSeerX Archives 2001 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.318.960 http://www.bio21.bas.bg/ipp/gapbfiles/v-27/01_1-2_43-53.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.318.960 http://www.bio21.bas.bg/ipp/gapbfiles/v-27/01_1-2_43-53.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.bio21.bas.bg/ipp/gapbfiles/v-27/01_1-2_43-53.pdf micropropagation rhizogenesis biochemical marker basic and acidic peroxidases text 2001 ftciteseerx 2016-09-04T00:13:25Z Summary. Peroxidases play a major role in several physiological processes. Specifically, in vitro activity of this enzyme was used as a marker to identify different rooting phases of Nothofagus. The study of peroxidase profile patterns during rhizogenesis in this genus could give additional information about this topic, to justify the use of peroxidase isoforms as a more specific biochemical marker than total peroxidase activity. This work gives information on qualitative and quantitative changes in isoperoxidase expression (basic and acidic peroxidases) during in vitro rooting of N. antarctica microshoots through native PAGE. Time course analysis of isoperoxidase activity during rhizogenesis allowed positive correlation with specific morphogenic stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four of the acidic peroxidases appeared during the induction stage of rooting. Variation in the number and relative activity of the isoperoxidases was found during different stages of adventitious rooting. The variation in the number and activity of isoperoxidases provides a good indicator of the processes involved in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases with auxin metabolism and the role of certain isoperoxidases in specific rooting processes are also discussed. Text Antarc* Antarctica Tierra del Fuego Unknown Argentina |
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ftciteseerx |
language |
English |
topic |
micropropagation rhizogenesis biochemical marker basic and acidic peroxidases |
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micropropagation rhizogenesis biochemical marker basic and acidic peroxidases Laboratorio De Fisiologia Vegetal G. Martinez Pastur Provincia de Tierra del Fuego (Argentina) |
topic_facet |
micropropagation rhizogenesis biochemical marker basic and acidic peroxidases |
description |
Summary. Peroxidases play a major role in several physiological processes. Specifically, in vitro activity of this enzyme was used as a marker to identify different rooting phases of Nothofagus. The study of peroxidase profile patterns during rhizogenesis in this genus could give additional information about this topic, to justify the use of peroxidase isoforms as a more specific biochemical marker than total peroxidase activity. This work gives information on qualitative and quantitative changes in isoperoxidase expression (basic and acidic peroxidases) during in vitro rooting of N. antarctica microshoots through native PAGE. Time course analysis of isoperoxidase activity during rhizogenesis allowed positive correlation with specific morphogenic stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four of the acidic peroxidases appeared during the induction stage of rooting. Variation in the number and relative activity of the isoperoxidases was found during different stages of adventitious rooting. The variation in the number and activity of isoperoxidases provides a good indicator of the processes involved in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases with auxin metabolism and the role of certain isoperoxidases in specific rooting processes are also discussed. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Laboratorio De Fisiologia Vegetal G. Martinez Pastur |
author_facet |
Laboratorio De Fisiologia Vegetal G. Martinez Pastur |
author_sort |
Laboratorio De Fisiologia Vegetal |
title |
Provincia de Tierra del Fuego (Argentina) |
title_short |
Provincia de Tierra del Fuego (Argentina) |
title_full |
Provincia de Tierra del Fuego (Argentina) |
title_fullStr |
Provincia de Tierra del Fuego (Argentina) |
title_full_unstemmed |
Provincia de Tierra del Fuego (Argentina) |
title_sort |
provincia de tierra del fuego (argentina) |
publishDate |
2001 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.318.960 http://www.bio21.bas.bg/ipp/gapbfiles/v-27/01_1-2_43-53.pdf |
geographic |
Argentina |
geographic_facet |
Argentina |
genre |
Antarc* Antarctica Tierra del Fuego |
genre_facet |
Antarc* Antarctica Tierra del Fuego |
op_source |
http://www.bio21.bas.bg/ipp/gapbfiles/v-27/01_1-2_43-53.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.318.960 http://www.bio21.bas.bg/ipp/gapbfiles/v-27/01_1-2_43-53.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766268751623225344 |