HSP_Harpagifer 1 The HSP70 heat shock response in the Antarctic fish Harpagifer antarcticus

• Main Authors: Melody S. Clark, Keiron P. P. Fraser, Gavin Burns, Lloyd S. Peck
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: Antarctic; Climate change; stenothermal; heat shock protein; marine HSP_Harpagifer 3; The Antarctic; Antarc*; antarcticus
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.318.2443; http://nora.nerc.ac.uk/3961/1/Harpy_HSP.pdf

Members of the HSP70 gene family comprising the constitutive (HSC70) and inducible (HSP70) genes, plus GRP78 (Glucose-regulated protein 78kDa) were surveyed for expression levels via Q-PCR after both an acute two-hour heat shock experiment and a time course assay in the Antarctic plunderfish Harpagifer antarcticus. In general, down regulation of all genes was observed during the course of the heat shock experiments. This thermally induced down regulation was particularly acute for the GRP78 gene, which at one time point was more than 100 fold down regulated. These results demonstrate the loss of the heat shock response in H. antarcticus, a basal member of the Notothenioidei. This finding is discussed with reference to the survival of Notothenioids during observed ocean warming and also the reorganisation of cellular protein mechanisms of species living in extreme environments.