Transcriptome profiling in AGD-affected Atlantic salmon 1 Transcriptome profiling the gills of amoebic gill disease (AGD)-affected Atlantic salmon (Salmo salar L.)- A role for tumor suppressor p53 in AGD-pathogenesis?

• Main Authors: Richard N. Morrison, Glenn A. Cooper, Ben F. Koop, Matthew L. Rise, Andrew R. Bridle, Mark B. Adams, Barbara F. Nowak
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: Atlantic salmon; Salmo salar
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.317.9406; http://physiolgenomics.physiology.org/content/early/2006/02/21/physiolgenomics.00320.2005.full.pdf

Neoparamoeba spp. are amphizoic amoebae with the capacity to colonize the gills of some marine fish, causing amoebic gill disease (AGD). Here, the gill tissue transcriptome response of Atlantic salmon (Salmo salar L.) to AGD is described. Tanks housing Atlantic salmon were inoculated with Neoparamoeba spp. and fish sampled at time points up to 8 days post-inoculation (p.i.). Gill tissues were taken from AGD-affected fish and a DNA microarray was used to compare global gene expression against tissues from AGD-unaffected fish. A total of 206 genes, representing 190 unique transcripts were reproducibly identified as up or downregulated in response to Neoparamoeba spp. infection. Informative transcripts having gene ontology (GO) biological process identifiers were grouped according to function. While a number of genes were placed into each category no distinct patterns were observed. One Atlantic salmon cDNA that was up-regulated in infected gill relative to non-infected gill at 114 and 189 h post-inoculation showed significant identity with the Xenopus, mouse and human anterior gradient-2 (AG-2) homologs. Two Atlantic