DOI 10.1007/s10096-010-1080-2 ARTICLE Evaluation of a fourth-generation latex agglutination test for the identification of Staphylococcus aureus
Abstract In this study, we evaluated a fourth-generation agglutination assay (Staph Plus; DiaMondiaL[DML]) for the rapid identification of Staphylococcus aureus. First, comparison with three third-generation assays (Slidex Staph Plus, bioMérieux; Staphaurex Plus, Murex Diagnostics; Pastorex Staph-Pl...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.292.539 2023-05-15T16:01:09+02:00 DOI 10.1007/s10096-010-1080-2 ARTICLE Evaluation of a fourth-generation latex agglutination test for the identification of Staphylococcus aureus J. A. J. W. Kluytmans The Pennsylvania State University CiteSeerX Archives 2010 application/zip http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.292.539 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.292.539 Metadata may be used without restrictions as long as the oai identifier remains attached to it. ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/53/4d/Eur_J_Clin_Microbiol_Infect_Dis_2011_Feb_13_30(2)_259-264.tar.gz text 2010 ftciteseerx 2016-01-07T21:38:09Z Abstract In this study, we evaluated a fourth-generation agglutination assay (Staph Plus; DiaMondiaL[DML]) for the rapid identification of Staphylococcus aureus. First, comparison with three third-generation assays (Slidex Staph Plus, bioMérieux; Staphaurex Plus, Murex Diagnostics; Pastorex Staph-Plus, Sanofi Diagnostics Pasteur) was performed on a predefined strain collection: 265 coagulase-negative staphylococci (CNS), 266 methicillinresistant S. aureus (MRSA) and 262 methicillin-susceptible S. aureus (MSSA) strains (“strain study”). Second, patient material-derived strains (883 CNS, 847 MSSA and 135 MRSA) were tested concurrently with both the DML and Slidex assays (“daily practice study”). In the strain study, the overall sensitivity and specificity of the DML, Slidex, Staphaurex and Pastorex assays were 99.2 % and 100%, 98.1 % and 100%, 95.2 % and 100%, and 98.2 % and 98.8%, respectively. Using the respective tests, the result was indeterminate in 0.0%, 0.6%, 0.4 % and 1.5 % of the strains. Overall, the sensitivity of the DML and Slidex assays were comparable in both sub-studies. However, in MRSA strains, the sensitivity of the DML assay was significantly lower than the Slidex assay. The specificity of the Slidex assay was significantly higher than the DML assay. However, the percentage of indeterminate results was much higher for the Slidex than the DML assay. In conclusion Text DML Unknown Pasteur ENVELOPE(140.099,140.099,-66.625,-66.625) |
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Abstract In this study, we evaluated a fourth-generation agglutination assay (Staph Plus; DiaMondiaL[DML]) for the rapid identification of Staphylococcus aureus. First, comparison with three third-generation assays (Slidex Staph Plus, bioMérieux; Staphaurex Plus, Murex Diagnostics; Pastorex Staph-Plus, Sanofi Diagnostics Pasteur) was performed on a predefined strain collection: 265 coagulase-negative staphylococci (CNS), 266 methicillinresistant S. aureus (MRSA) and 262 methicillin-susceptible S. aureus (MSSA) strains (“strain study”). Second, patient material-derived strains (883 CNS, 847 MSSA and 135 MRSA) were tested concurrently with both the DML and Slidex assays (“daily practice study”). In the strain study, the overall sensitivity and specificity of the DML, Slidex, Staphaurex and Pastorex assays were 99.2 % and 100%, 98.1 % and 100%, 95.2 % and 100%, and 98.2 % and 98.8%, respectively. Using the respective tests, the result was indeterminate in 0.0%, 0.6%, 0.4 % and 1.5 % of the strains. Overall, the sensitivity of the DML and Slidex assays were comparable in both sub-studies. However, in MRSA strains, the sensitivity of the DML assay was significantly lower than the Slidex assay. The specificity of the Slidex assay was significantly higher than the DML assay. However, the percentage of indeterminate results was much higher for the Slidex than the DML assay. In conclusion |
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The Pennsylvania State University CiteSeerX Archives |
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J. A. J. W. Kluytmans |
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J. A. J. W. Kluytmans DOI 10.1007/s10096-010-1080-2 ARTICLE Evaluation of a fourth-generation latex agglutination test for the identification of Staphylococcus aureus |
author_facet |
J. A. J. W. Kluytmans |
author_sort |
J. A. J. W. Kluytmans |
title |
DOI 10.1007/s10096-010-1080-2 ARTICLE Evaluation of a fourth-generation latex agglutination test for the identification of Staphylococcus aureus |
title_short |
DOI 10.1007/s10096-010-1080-2 ARTICLE Evaluation of a fourth-generation latex agglutination test for the identification of Staphylococcus aureus |
title_full |
DOI 10.1007/s10096-010-1080-2 ARTICLE Evaluation of a fourth-generation latex agglutination test for the identification of Staphylococcus aureus |
title_fullStr |
DOI 10.1007/s10096-010-1080-2 ARTICLE Evaluation of a fourth-generation latex agglutination test for the identification of Staphylococcus aureus |
title_full_unstemmed |
DOI 10.1007/s10096-010-1080-2 ARTICLE Evaluation of a fourth-generation latex agglutination test for the identification of Staphylococcus aureus |
title_sort |
doi 10.1007/s10096-010-1080-2 article evaluation of a fourth-generation latex agglutination test for the identification of staphylococcus aureus |
publishDate |
2010 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.292.539 |
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ENVELOPE(140.099,140.099,-66.625,-66.625) |
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Pasteur |
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Pasteur |
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DML |
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DML |
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ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/53/4d/Eur_J_Clin_Microbiol_Infect_Dis_2011_Feb_13_30(2)_259-264.tar.gz |
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http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.292.539 |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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