A Voltage-Gated H + Channel Underlying pH Homeostasis
Marine coccolithophorid phytoplankton are major producers of biogenic calcite, playing a significant role in the global carbon cycle. Predicting the impacts of ocean acidification on coccolithophore calcification has received much recent attention and requires improved knowledge of cellular calcific...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.291.3138 2023-05-15T17:51:22+02:00 A Voltage-Gated H + Channel Underlying pH Homeostasis In Calcifying Coccolithophores Alison R. Taylor Abdul Chrachri Glen Wheeler Helen Goddard Colin Brownlee The Pennsylvania State University CiteSeerX Archives application/zip http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.291.3138 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.291.3138 Metadata may be used without restrictions as long as the oai identifier remains attached to it. ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/5a/5e/PLoS_Biol_2011_Jun_21_9(6)_e1001085.tar.gz text ftciteseerx 2016-01-07T21:34:53Z Marine coccolithophorid phytoplankton are major producers of biogenic calcite, playing a significant role in the global carbon cycle. Predicting the impacts of ocean acidification on coccolithophore calcification has received much recent attention and requires improved knowledge of cellular calcification mechanisms. Uniquely amongst calcifying organisms, coccolithophores produce calcified scales (coccoliths) in an intracellular compartment and secrete them to the cell surface, requiring large transcellular ionic fluxes to support calcification. In particular, intracellular calcite precipitation using HCO 3 2 as the substrate generates equimolar quantities of H + that must be rapidly removed to prevent cytoplasmic acidification. We have used electrophysiological approaches to identify a plasma membrane voltage-gated H + conductance in Coccolithus pelagicus ssp braarudii with remarkably similar biophysical and functional properties to those found in metazoans. We show that both C. pelagicus and Emiliania huxleyi possess homologues of metazoan H v1H + channels, which function as voltagegated H + channels when expressed in heterologous systems. Homologues of the coccolithophore H + channels were also identified in a diversity of eukaryotes, suggesting a wide range of cellular roles for the Hv1 class of proteins. Using single cell imaging, we demonstrate that the coccolithophore H + conductance mediates rapid H + efflux and plays an important role in pH homeostasis in calcifying cells. The results demonstrate a novel cellular role for voltage gated H + channels and provide Text Ocean acidification Unknown |
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English |
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Marine coccolithophorid phytoplankton are major producers of biogenic calcite, playing a significant role in the global carbon cycle. Predicting the impacts of ocean acidification on coccolithophore calcification has received much recent attention and requires improved knowledge of cellular calcification mechanisms. Uniquely amongst calcifying organisms, coccolithophores produce calcified scales (coccoliths) in an intracellular compartment and secrete them to the cell surface, requiring large transcellular ionic fluxes to support calcification. In particular, intracellular calcite precipitation using HCO 3 2 as the substrate generates equimolar quantities of H + that must be rapidly removed to prevent cytoplasmic acidification. We have used electrophysiological approaches to identify a plasma membrane voltage-gated H + conductance in Coccolithus pelagicus ssp braarudii with remarkably similar biophysical and functional properties to those found in metazoans. We show that both C. pelagicus and Emiliania huxleyi possess homologues of metazoan H v1H + channels, which function as voltagegated H + channels when expressed in heterologous systems. Homologues of the coccolithophore H + channels were also identified in a diversity of eukaryotes, suggesting a wide range of cellular roles for the Hv1 class of proteins. Using single cell imaging, we demonstrate that the coccolithophore H + conductance mediates rapid H + efflux and plays an important role in pH homeostasis in calcifying cells. The results demonstrate a novel cellular role for voltage gated H + channels and provide |
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The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
In Calcifying Coccolithophores Alison R. Taylor Abdul Chrachri Glen Wheeler Helen Goddard Colin Brownlee |
spellingShingle |
In Calcifying Coccolithophores Alison R. Taylor Abdul Chrachri Glen Wheeler Helen Goddard Colin Brownlee A Voltage-Gated H + Channel Underlying pH Homeostasis |
author_facet |
In Calcifying Coccolithophores Alison R. Taylor Abdul Chrachri Glen Wheeler Helen Goddard Colin Brownlee |
author_sort |
In Calcifying Coccolithophores |
title |
A Voltage-Gated H + Channel Underlying pH Homeostasis |
title_short |
A Voltage-Gated H + Channel Underlying pH Homeostasis |
title_full |
A Voltage-Gated H + Channel Underlying pH Homeostasis |
title_fullStr |
A Voltage-Gated H + Channel Underlying pH Homeostasis |
title_full_unstemmed |
A Voltage-Gated H + Channel Underlying pH Homeostasis |
title_sort |
voltage-gated h + channel underlying ph homeostasis |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.291.3138 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/5a/5e/PLoS_Biol_2011_Jun_21_9(6)_e1001085.tar.gz |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.291.3138 |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766158493886185472 |