www.mdpi.com/journal/ijms Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant
Abstract: Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was dev...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.290.9594 2023-05-15T15:34:18+02:00 www.mdpi.com/journal/ijms Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-elastin-like Polypeptide Fusions Hoang Trong Phan Udo Conrad The Pennsylvania State University CiteSeerX Archives 2011 application/zip http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.290.9594 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.290.9594 Metadata may be used without restrictions as long as the oai identifier remains attached to it. ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/42/d6/Int_J_Mol_Sci_2011_Apr_29_12(5)_2808-2821.tar.gz transgenic plants avian influenza H5N1 nanobody ELPylation Inverse Transition Cycling text 2011 ftciteseerx 2016-01-07T21:34:06Z Abstract: Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to recover the ELPylated antigens and antibodies from plant material. The functionality of both the ELPylated neuraminidase and an ELPylated nanobody was demonstrated. Text Avian flu Unknown |
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ftciteseerx |
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English |
topic |
transgenic plants avian influenza H5N1 nanobody ELPylation Inverse Transition Cycling |
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transgenic plants avian influenza H5N1 nanobody ELPylation Inverse Transition Cycling Protein-elastin-like Polypeptide Fusions Hoang Trong Phan Udo Conrad www.mdpi.com/journal/ijms Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant |
topic_facet |
transgenic plants avian influenza H5N1 nanobody ELPylation Inverse Transition Cycling |
description |
Abstract: Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to recover the ELPylated antigens and antibodies from plant material. The functionality of both the ELPylated neuraminidase and an ELPylated nanobody was demonstrated. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Protein-elastin-like Polypeptide Fusions Hoang Trong Phan Udo Conrad |
author_facet |
Protein-elastin-like Polypeptide Fusions Hoang Trong Phan Udo Conrad |
author_sort |
Protein-elastin-like Polypeptide Fusions |
title |
www.mdpi.com/journal/ijms Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant |
title_short |
www.mdpi.com/journal/ijms Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant |
title_full |
www.mdpi.com/journal/ijms Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant |
title_fullStr |
www.mdpi.com/journal/ijms Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant |
title_full_unstemmed |
www.mdpi.com/journal/ijms Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant |
title_sort |
www.mdpi.com/journal/ijms membrane-based inverse transition cycling: an improved means for purifying plant-derived recombinant |
publishDate |
2011 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.290.9594 |
genre |
Avian flu |
genre_facet |
Avian flu |
op_source |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/42/d6/Int_J_Mol_Sci_2011_Apr_29_12(5)_2808-2821.tar.gz |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.290.9594 |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766364745881878528 |