www.mdpi.com/journal/ijms Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant

Abstract: Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was dev...

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Bibliographic Details
Main Authors: Protein-elastin-like Polypeptide Fusions, Hoang Trong Phan, Udo Conrad
Other Authors: The Pennsylvania State University CiteSeerX Archives
Format: Text
Language:English
Published: 2011
Subjects:
transgenic plants
avian influenza
H5N1
nanobody
ELPylation
Inverse Transition Cycling
Avian flu
Online Access:http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.290.9594
Description
Summary:Abstract: Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to recover the ELPylated antigens and antibodies from plant material. The functionality of both the ELPylated neuraminidase and an ELPylated nanobody was demonstrated.

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