Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey
Abstract: Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for e...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.290.7854 2023-05-15T13:36:05+02:00 Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey Lidiane S. Araújo Edna Kagohara Thaís P. Garcia Vivian H. Pellizari Ro H. Andrade The Pennsylvania State University CiteSeerX Archives 2011 application/zip http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.290.7854 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.290.7854 Metadata may be used without restrictions as long as the oai identifier remains attached to it. ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/38/48/Mar_Drugs_2011_May_24_9(5)_889-905.tar.gz oxidoreductases chiral alcohols psychrophile psychrotroph oxidation text 2011 ftciteseerx 2016-01-07T21:33:36Z Abstract: Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze the (S)-enantiomer oxidation from racemic alcohols to their corresponding ketones. Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity. These new bacteria strains were selected for optimization study, in which the (RS)-1-(4-methyl-phenyl)ethanol oxidation was evaluated in several reaction conditions. From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C. We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph. Mar. Drugs 2011, 9 890 Text Antarc* Antarctic Antarctic Peninsula Unknown Antarctic Antarctic Peninsula |
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English |
topic |
oxidoreductases chiral alcohols psychrophile psychrotroph oxidation |
spellingShingle |
oxidoreductases chiral alcohols psychrophile psychrotroph oxidation Lidiane S. Araújo Edna Kagohara Thaís P. Garcia Vivian H. Pellizari Ro H. Andrade Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
topic_facet |
oxidoreductases chiral alcohols psychrophile psychrotroph oxidation |
description |
Abstract: Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze the (S)-enantiomer oxidation from racemic alcohols to their corresponding ketones. Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity. These new bacteria strains were selected for optimization study, in which the (RS)-1-(4-methyl-phenyl)ethanol oxidation was evaluated in several reaction conditions. From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C. We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph. Mar. Drugs 2011, 9 890 |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Lidiane S. Araújo Edna Kagohara Thaís P. Garcia Vivian H. Pellizari Ro H. Andrade |
author_facet |
Lidiane S. Araújo Edna Kagohara Thaís P. Garcia Vivian H. Pellizari Ro H. Andrade |
author_sort |
Lidiane S. Araújo |
title |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_short |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_full |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_fullStr |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_full_unstemmed |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_sort |
screening of microorganisms producing cold-active oxidoreductases to be applied in enantioselective alcohol oxidation. an antarctic survey |
publishDate |
2011 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.290.7854 |
geographic |
Antarctic Antarctic Peninsula |
geographic_facet |
Antarctic Antarctic Peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula |
genre_facet |
Antarc* Antarctic Antarctic Peninsula |
op_source |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/38/48/Mar_Drugs_2011_May_24_9(5)_889-905.tar.gz |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.290.7854 |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766074091158110208 |