Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study
Experimental infection with Pseudomonas anguilliseptica was performed both by intraperitoneal (i.p.) and bath route on juvenile turbot (Psetta maxima) in order to evaluate the pathology induced. Turbot was found to be sensitive to i.p. challenge (1.7x10 6 CFU/fish) but no to bath exposure.The i.p. c...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.289.6372 2023-05-15T18:41:06+02:00 Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study G. E. Magi S. Lopez-romalde B. Magariños J. Lamas A. E. Toranzo J. L. Romalde The Pennsylvania State University CiteSeerX Archives application/zip http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.289.6372 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.289.6372 Metadata may be used without restrictions as long as the oai identifier remains attached to it. ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/dc/24/Eur_J_Histochem_2009_Jun_29_53(2)_e9.tar.gz Correspondence Gianenrico Enrico Magi text ftciteseerx 2016-01-07T21:27:29Z Experimental infection with Pseudomonas anguilliseptica was performed both by intraperitoneal (i.p.) and bath route on juvenile turbot (Psetta maxima) in order to evaluate the pathology induced. Turbot was found to be sensitive to i.p. challenge (1.7x10 6 CFU/fish) but no to bath exposure.The i.p. challenge induced septicaemic infection and mortality. Externally, moribund fish showed distended abdomen and pale areas at day 9. The gross pathological internal signs present were abundant ascitic fluid in the peritoneal cavity, pale and enlarged spleen, pale and friable liver, and congestive and dilated gut with yellowish exudates. On histopathological examination, bacterial invasion was common in all the tissues studied but the most prominent pathological changes were observed in gut, spleen and kidney after 7 day with features of necrosis. The immunohistochemical findings support the widespread localization of the bacteria after the i.p. injection since the P. anguilliseptica was detected in spleen from day 1 post injection, in liver, kidney and gut from day 4, in muscle from day 7 and in brain from day 9. The difficulties in infecting healthy fish by bath challenge can be explained by the opportunistic nature of this pathogen. Text Turbot Unknown |
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Correspondence Gianenrico Enrico Magi |
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Correspondence Gianenrico Enrico Magi G. E. Magi S. Lopez-romalde B. Magariños J. Lamas A. E. Toranzo J. L. Romalde Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
topic_facet |
Correspondence Gianenrico Enrico Magi |
description |
Experimental infection with Pseudomonas anguilliseptica was performed both by intraperitoneal (i.p.) and bath route on juvenile turbot (Psetta maxima) in order to evaluate the pathology induced. Turbot was found to be sensitive to i.p. challenge (1.7x10 6 CFU/fish) but no to bath exposure.The i.p. challenge induced septicaemic infection and mortality. Externally, moribund fish showed distended abdomen and pale areas at day 9. The gross pathological internal signs present were abundant ascitic fluid in the peritoneal cavity, pale and enlarged spleen, pale and friable liver, and congestive and dilated gut with yellowish exudates. On histopathological examination, bacterial invasion was common in all the tissues studied but the most prominent pathological changes were observed in gut, spleen and kidney after 7 day with features of necrosis. The immunohistochemical findings support the widespread localization of the bacteria after the i.p. injection since the P. anguilliseptica was detected in spleen from day 1 post injection, in liver, kidney and gut from day 4, in muscle from day 7 and in brain from day 9. The difficulties in infecting healthy fish by bath challenge can be explained by the opportunistic nature of this pathogen. |
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The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
G. E. Magi S. Lopez-romalde B. Magariños J. Lamas A. E. Toranzo J. L. Romalde |
author_facet |
G. E. Magi S. Lopez-romalde B. Magariños J. Lamas A. E. Toranzo J. L. Romalde |
author_sort |
G. E. Magi |
title |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_short |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_full |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_fullStr |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_full_unstemmed |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_sort |
experimental pseudomonas anguilliseptica infection in turbot psetta maxima (l.): a histopathological and immunohistochemical study |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.289.6372 |
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Turbot |
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Turbot |
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ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/dc/24/Eur_J_Histochem_2009_Jun_29_53(2)_e9.tar.gz |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.289.6372 |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766230602184392704 |