Evidence of an Antimicrobial-Immunomodulatory Role of Atlantic Salmon Cathelicidins during Infection with
Cathelicidins are a family of antimicrobial peptides that act as effector molecules of the innate immune system with broadspectrum antimicrobial properties. These evolutionary conserved cationic host-defence peptides are integral components of the immune response of fish, which are generally believe...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.289.4635 2023-05-15T15:31:38+02:00 Evidence of an Antimicrobial-Immunomodulatory Role of Atlantic Salmon Cathelicidins during Infection with Yersinia Ruckeri Andrew Bridle* Elizabeth Nosworthy The Pennsylvania State University CiteSeerX Archives application/zip http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.289.4635 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.289.4635 Metadata may be used without restrictions as long as the oai identifier remains attached to it. ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/ec/ad/PLoS_One_2011_Aug_9_6(8)_e23417.tar.gz text ftciteseerx 2016-01-07T21:26:58Z Cathelicidins are a family of antimicrobial peptides that act as effector molecules of the innate immune system with broadspectrum antimicrobial properties. These evolutionary conserved cationic host-defence peptides are integral components of the immune response of fish, which are generally believed to rely heavily on innate immune defences to invading pathogens. In this study we showed that Atlantic salmon cathelicidin 1 and 2 (asCATH1 and asCATH2) stimulated peripheral blood leukocytes increasing the transcription of the chemokine interleukin-8. Further, functional differences were identified between the two cathelicidins. In the presence of serum, asCATH1 displayed greatly diminished host haemolytic activity, while the constitutively expressed asCATH2 had no haemolytic activity with or without serum. These findings support our hypothesis that fish cathelicidins exert their primary antimicrobial action at the site of pathogen invasion such as epithelial surfaces. Further, we hypothesise that like their mammalian counterparts in the presence of serum they act as mediators of the innate and adaptive immune response via the release of cytokines thus indirectly protecting against a variety of pathogens. We highlight the importance of this immunomodulatory role from the involvement of asCATHs during an infection with the fish pathogen Yersinia ruckeri. While we were able to demonstrate in vitro that asCATH1 and 2, possessed direct microbicidal activity against the fish pathogen, Vibrio anguillarum, and a common gram negative bacterium, Escherichia coli, little or no bactericidal activity was found against Y. ruckeri. The contribution of either asCATH in the Text Atlantic salmon Unknown |
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Cathelicidins are a family of antimicrobial peptides that act as effector molecules of the innate immune system with broadspectrum antimicrobial properties. These evolutionary conserved cationic host-defence peptides are integral components of the immune response of fish, which are generally believed to rely heavily on innate immune defences to invading pathogens. In this study we showed that Atlantic salmon cathelicidin 1 and 2 (asCATH1 and asCATH2) stimulated peripheral blood leukocytes increasing the transcription of the chemokine interleukin-8. Further, functional differences were identified between the two cathelicidins. In the presence of serum, asCATH1 displayed greatly diminished host haemolytic activity, while the constitutively expressed asCATH2 had no haemolytic activity with or without serum. These findings support our hypothesis that fish cathelicidins exert their primary antimicrobial action at the site of pathogen invasion such as epithelial surfaces. Further, we hypothesise that like their mammalian counterparts in the presence of serum they act as mediators of the innate and adaptive immune response via the release of cytokines thus indirectly protecting against a variety of pathogens. We highlight the importance of this immunomodulatory role from the involvement of asCATHs during an infection with the fish pathogen Yersinia ruckeri. While we were able to demonstrate in vitro that asCATH1 and 2, possessed direct microbicidal activity against the fish pathogen, Vibrio anguillarum, and a common gram negative bacterium, Escherichia coli, little or no bactericidal activity was found against Y. ruckeri. The contribution of either asCATH in the |
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The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Yersinia Ruckeri Andrew Bridle* Elizabeth Nosworthy |
spellingShingle |
Yersinia Ruckeri Andrew Bridle* Elizabeth Nosworthy Evidence of an Antimicrobial-Immunomodulatory Role of Atlantic Salmon Cathelicidins during Infection with |
author_facet |
Yersinia Ruckeri Andrew Bridle* Elizabeth Nosworthy |
author_sort |
Yersinia Ruckeri |
title |
Evidence of an Antimicrobial-Immunomodulatory Role of Atlantic Salmon Cathelicidins during Infection with |
title_short |
Evidence of an Antimicrobial-Immunomodulatory Role of Atlantic Salmon Cathelicidins during Infection with |
title_full |
Evidence of an Antimicrobial-Immunomodulatory Role of Atlantic Salmon Cathelicidins during Infection with |
title_fullStr |
Evidence of an Antimicrobial-Immunomodulatory Role of Atlantic Salmon Cathelicidins during Infection with |
title_full_unstemmed |
Evidence of an Antimicrobial-Immunomodulatory Role of Atlantic Salmon Cathelicidins during Infection with |
title_sort |
evidence of an antimicrobial-immunomodulatory role of atlantic salmon cathelicidins during infection with |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.289.4635 |
genre |
Atlantic salmon |
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Atlantic salmon |
op_source |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/ec/ad/PLoS_One_2011_Aug_9_6(8)_e23417.tar.gz |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.289.4635 |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766362172376481792 |