Cloning and Sequencing of Protein Kinase CDNA from Harbor
Protein kinases (PKs) play critical roles in signal transduction and activation of lymphocytes. The identification of PK genes provides a tool for understanding mechanisms of immunotoxic xenobiotics. As part of a larger study investigating persistent organic pollutants in the harbor seal and their p...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.283.3081 2023-05-15T16:33:02+02:00 Cloning and Sequencing of Protein Kinase CDNA from Harbor Seal (phoca Vitulina Lymphocytes Jennifer C. C. Neale Thomas P. Kenny M. Eric Gershwin The Pennsylvania State University CiteSeerX Archives application/zip http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.283.3081 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.283.3081 Metadata may be used without restrictions as long as the oai identifier remains attached to it. ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/ff/b7/Clin_Dev_Immunol_2004_Jun_11(2)_157-163.tar.gz Harbor seal Phoca vitulina Protein (tyrosine) kinase Lymphocyte activation and text ftciteseerx 2016-01-07T21:10:28Z Protein kinases (PKs) play critical roles in signal transduction and activation of lymphocytes. The identification of PK genes provides a tool for understanding mechanisms of immunotoxic xenobiotics. As part of a larger study investigating persistent organic pollutants in the harbor seal and their possible immunomodulatory actions, we sequenced harbor seal cDNA fragments encoding PKs. The procedure, using degenerate primers based on conserved motifs of human protein tyrosine kinases (PTKs), successfully amplified nine phocid PK gene fragments with high homology to human and rodent orthologs. We identified eight PTKs and one dual (serine/threonine and tyrosine) kinase. Among these were several PKs important in early signaling events through the B- and T-cell receptors (FYN, LYN, ITK and SYK) and a MAP kinase involved in downstream signal transduction. V-FGR, RET and DDR2 were also expressed. Sequential activation of protein kinases ultimately induces gene transcription leading to the proliferation and differentiation of lymphocytes critical to adaptive immunity. PKs are potential targets of bioactive xenobiotics, including persistent organic pollutants of the marine environment; characterization of these molecules in the harbor seal provides a foundation for further research illuminating mechanisms of action of contaminants speculated to contribute to large-scale dieoffs of marine mammals via immunosuppression. Text harbor seal Phoca vitulina Unknown Fyn ENVELOPE(20.735,20.735,69.763,69.763) |
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English |
topic |
Harbor seal Phoca vitulina Protein (tyrosine) kinase Lymphocyte activation and |
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Harbor seal Phoca vitulina Protein (tyrosine) kinase Lymphocyte activation and Seal (phoca Vitulina Lymphocytes Jennifer C. C. Neale Thomas P. Kenny M. Eric Gershwin Cloning and Sequencing of Protein Kinase CDNA from Harbor |
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Harbor seal Phoca vitulina Protein (tyrosine) kinase Lymphocyte activation and |
description |
Protein kinases (PKs) play critical roles in signal transduction and activation of lymphocytes. The identification of PK genes provides a tool for understanding mechanisms of immunotoxic xenobiotics. As part of a larger study investigating persistent organic pollutants in the harbor seal and their possible immunomodulatory actions, we sequenced harbor seal cDNA fragments encoding PKs. The procedure, using degenerate primers based on conserved motifs of human protein tyrosine kinases (PTKs), successfully amplified nine phocid PK gene fragments with high homology to human and rodent orthologs. We identified eight PTKs and one dual (serine/threonine and tyrosine) kinase. Among these were several PKs important in early signaling events through the B- and T-cell receptors (FYN, LYN, ITK and SYK) and a MAP kinase involved in downstream signal transduction. V-FGR, RET and DDR2 were also expressed. Sequential activation of protein kinases ultimately induces gene transcription leading to the proliferation and differentiation of lymphocytes critical to adaptive immunity. PKs are potential targets of bioactive xenobiotics, including persistent organic pollutants of the marine environment; characterization of these molecules in the harbor seal provides a foundation for further research illuminating mechanisms of action of contaminants speculated to contribute to large-scale dieoffs of marine mammals via immunosuppression. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Seal (phoca Vitulina Lymphocytes Jennifer C. C. Neale Thomas P. Kenny M. Eric Gershwin |
author_facet |
Seal (phoca Vitulina Lymphocytes Jennifer C. C. Neale Thomas P. Kenny M. Eric Gershwin |
author_sort |
Seal (phoca Vitulina Lymphocytes |
title |
Cloning and Sequencing of Protein Kinase CDNA from Harbor |
title_short |
Cloning and Sequencing of Protein Kinase CDNA from Harbor |
title_full |
Cloning and Sequencing of Protein Kinase CDNA from Harbor |
title_fullStr |
Cloning and Sequencing of Protein Kinase CDNA from Harbor |
title_full_unstemmed |
Cloning and Sequencing of Protein Kinase CDNA from Harbor |
title_sort |
cloning and sequencing of protein kinase cdna from harbor |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.283.3081 |
long_lat |
ENVELOPE(20.735,20.735,69.763,69.763) |
geographic |
Fyn |
geographic_facet |
Fyn |
genre |
harbor seal Phoca vitulina |
genre_facet |
harbor seal Phoca vitulina |
op_source |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/ff/b7/Clin_Dev_Immunol_2004_Jun_11(2)_157-163.tar.gz |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.283.3081 |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766022760094498816 |