T cell receptor (TCR)mediated repertoire selection and loss of TCR vbeta diversity during the initiation of a CD4(+) T cell response
We recently described a novel way to isolate populations of antigen-reactive CD4 � T cells with a wide range of reactivity to a specific antigen, using immunization with a fixed dose of nominal antigen and FACS ® sorting by CD4 high expression. Phenotypic, FACS ® , functional, antibody inhibition, a...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.282.2508 2023-05-15T18:26:47+02:00 T cell receptor (TCR)mediated repertoire selection and loss of TCR vbeta diversity during the initiation of a CD4(+) T cell response Marcella Fassò Niroshana An Frances Crawford C. Garrison Fathman William M. Ridgway The Pennsylvania State University CiteSeerX Archives 2000 application/zip http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.282.2508 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.282.2508 Metadata may be used without restrictions as long as the oai identifier remains attached to it. ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/a6/d8/J_Exp_Med_2000_Dec_18_192(12)_1719-1730.tar.gz text 2000 ftciteseerx 2016-01-07T21:07:46Z We recently described a novel way to isolate populations of antigen-reactive CD4 � T cells with a wide range of reactivity to a specific antigen, using immunization with a fixed dose of nominal antigen and FACS ® sorting by CD4 high expression. Phenotypic, FACS ® , functional, antibody inhibition, and major histocompatibility complex–peptide tetramer analyses, as well as T cell receptor V � sequence analyses, of the antigen-specific CD4 high T cell populations demonstrated that a diverse sperm whale myoglobin 110–121–reactive CD4 � T cell repertoire was activated at the beginning (day 3 after immunization) of the immune response. Within 6 d of immunization, lower affinity clones were lost from the responding population, leaving an expanded population of oligoclonal, intermediate affinity (and residual high affinity) T cells. This T cell subset persisted for at least 4 wk after immunization and dominated the secondary immune response. These data provide evidence that CD4 � T cell repertoire selection occurs early in the immune response in vivo and suggest that persistence and expansion of a population of oligoclonal, intermediate affinity T cells is involved in CD4 � T cell memory. Key words: T cells • clonal selection • T cell receptor • major histocompatibility complex class II tetramers • immune response Text Sperm whale Unknown |
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description |
We recently described a novel way to isolate populations of antigen-reactive CD4 � T cells with a wide range of reactivity to a specific antigen, using immunization with a fixed dose of nominal antigen and FACS ® sorting by CD4 high expression. Phenotypic, FACS ® , functional, antibody inhibition, and major histocompatibility complex–peptide tetramer analyses, as well as T cell receptor V � sequence analyses, of the antigen-specific CD4 high T cell populations demonstrated that a diverse sperm whale myoglobin 110–121–reactive CD4 � T cell repertoire was activated at the beginning (day 3 after immunization) of the immune response. Within 6 d of immunization, lower affinity clones were lost from the responding population, leaving an expanded population of oligoclonal, intermediate affinity (and residual high affinity) T cells. This T cell subset persisted for at least 4 wk after immunization and dominated the secondary immune response. These data provide evidence that CD4 � T cell repertoire selection occurs early in the immune response in vivo and suggest that persistence and expansion of a population of oligoclonal, intermediate affinity T cells is involved in CD4 � T cell memory. Key words: T cells • clonal selection • T cell receptor • major histocompatibility complex class II tetramers • immune response |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Marcella Fassò Niroshana An Frances Crawford C. Garrison Fathman William M. Ridgway |
spellingShingle |
Marcella Fassò Niroshana An Frances Crawford C. Garrison Fathman William M. Ridgway T cell receptor (TCR)mediated repertoire selection and loss of TCR vbeta diversity during the initiation of a CD4(+) T cell response |
author_facet |
Marcella Fassò Niroshana An Frances Crawford C. Garrison Fathman William M. Ridgway |
author_sort |
Marcella Fassò |
title |
T cell receptor (TCR)mediated repertoire selection and loss of TCR vbeta diversity during the initiation of a CD4(+) T cell response |
title_short |
T cell receptor (TCR)mediated repertoire selection and loss of TCR vbeta diversity during the initiation of a CD4(+) T cell response |
title_full |
T cell receptor (TCR)mediated repertoire selection and loss of TCR vbeta diversity during the initiation of a CD4(+) T cell response |
title_fullStr |
T cell receptor (TCR)mediated repertoire selection and loss of TCR vbeta diversity during the initiation of a CD4(+) T cell response |
title_full_unstemmed |
T cell receptor (TCR)mediated repertoire selection and loss of TCR vbeta diversity during the initiation of a CD4(+) T cell response |
title_sort |
t cell receptor (tcr)mediated repertoire selection and loss of tcr vbeta diversity during the initiation of a cd4(+) t cell response |
publishDate |
2000 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.282.2508 |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/a6/d8/J_Exp_Med_2000_Dec_18_192(12)_1719-1730.tar.gz |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.282.2508 |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766208761595166720 |